DMSO Modified Acid Fast Decolorizer

Use Thermo Scientific™ Remel™ DMSO Modified Acid Fast Decolorizer with DMSO Stain Kit (R246303) to detect and identify acid fast organisms (i.e., Cryptosporidium, Isospora, and Cyclospora spp.) in fecal material. It is a modification of Kinyoun method and may be also used for detecting acid-fast bacilli (Mycobacterium spp.) in sputum, urine, gastric lavage, other body fluids and tissues.

Cryptosporidium is a significant enteric pathogen that causes acute diarrhea in immunodeficient patients and transient diarrhea in immunocompetent individuals, particularly youngsters^1-5. The need to facilitate appropriate treatment for infected patients has led to the development of rapid methods for detection of these organisms.

The DMSO Modified acid-fast stain is a rapid and permanent differential staining procedure for detection and identification of acid-fast organisms. DMSO method is reliable and consistent in detecting cryptosporidiosis. This method also detects other pathogens such as Cyclospora and Isospora⁶.

Dimethylsulfoxide (DMSO) added to carbolfuchsin enhances penetration of acid-fast organisms by the stain and eliminates the need for heat or steam in the staining process. The stain is removed from background material by the decolorizing solution.

• Ready-to-use – Liquid reagent
• Rapid Test – Faster and easier to perform and reduces health risks associated with prolonged handling of contaminated material
• Easy-to-interpret – Fast green counterstains the background material pale green. The oocysts of Cryptosporidium, Isospora, and Cyclospora stain pink to red to purple and are easily distinguishable against the pale green background of the counterstain
• Easy-to-store – The product is stored at room temperature

This reagent contains 10% Sulfuric acid supplied in 250mL bottles.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.