Invitrogen™

FxCycle™ Violet Ready Flow™ Reagent

Catalog number:  R37166

Related applications:

Cell Analysis

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Description

FxCycle™ Violet Ready Flow™ Reagent is a bright, easy-to-use dsDNA stain for cell cycle analysis in fixed cells using flow cytometry. It is excitable by both UV and 405 nm lasers with an emission peak at 461 nm.

• Stable at room temperature
• Convenient, ready-to-use format—no need to dilute, weigh, or pipette
• Rapid staining of DNA in fixed cells
• No RNase treatment required

FxCycle Violet Ready Flow Reagent is a ready-to-use, direct-to-sample DNA stain. Add 2 drops from the dropper bottle directly into 1 x 106 fixed cells, incubate, and analyze.

As a cell-impermeant dye, FxCycle Violet Ready Flow Reagent enters the cell after fixation and binds double-stranded DNA specifically, eliminating the need for RNase treatment. The dye can be detected when excited with a UV or 405 nm laser, and the emission captured at a peak of 461 nm. FxCycle Violet Ready Flow Reagent is an ideal choice for DNA content analysis and can be included in multi-color cell-cycle studies.

Specifications

Cell Permeability: Cell-Impermeant
Excitation/Emission: 358⁄461
Product Line: FxCycle™ Ready Flow
Sub Cellular Localization: Nucleus
Color: Blue

Contents & storage

2 dropper bottles, store at room temperature