Invitrogen™

Annexin V Ready Flow Conjugates for Apoptosis Detection

Catalog number: R37177
Invitrogen™

Annexin V Ready Flow Conjugates for Apoptosis Detection

Catalog number: R37177
Enjoy the convenience of ready-to-use Annexin V Ready Flow stand-alone conjugate stains, which offer up to 100-fold fluorescence signal intensity between apoptotic and non-apoptotic cells using flow cytometry. There is no need to dilute, weigh, or pipette the conjugate stains; simply add one drop into cells, incubate, and analyze.
 
Catalog Number
R37177
Unit Size
120 reactions
Conjugate
Pacific Blue
Excitation/Emission
410/455
Color
Blue
Price (USD)
Price: 227.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionAnnexin V Pacific Blue Ready Flow Conjugate
Kit Contents2 dropper bottles, store at room temperature
Product TypeReady Flow Conjugate
Flow Cytometer Laser LinesViolet
Excitation/Emission410/455
ColorBlue
Shipping ConditionRoom Temperature
ConjugatePacific Blue
For Use With (Equipment)Flow Cytometer
Unit Size120 reactions
Catalog Number
Unit Size
120 reactions
Conjugate
Alexa Fluor 488
Excitation/Emission
495/519
Color
Green
Price (USD)
Price: 227.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionAnnexin V Alexa Fluor 488 Ready Flow Conjugate
Kit Contents2 dropper bottles, store at room temperature
Product TypeReady Flow Conjugate
Flow Cytometer Laser LinesBlue
Excitation/Emission495/519
ColorGreen
Shipping ConditionRoom Temperature
ConjugateAlexa Fluor 488
For Use With (Equipment)Flow Cytometer
Unit Size120 reactions
Catalog Number
Unit Size
120 reactions
Conjugate
Alexa Fluor 647
Excitation/Emission
650/665
Color
Far-Red
Price (USD)
Price: 319.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionAnnexin V Alexa Fluor 647 Ready Flow Conjugate
Kit Contents2 dropper bottles, store at room temperature
Product TypeReady Flow Conjugate
Flow Cytometer Laser LinesRed
Excitation/Emission650/665
ColorFar-Red
Shipping ConditionRoom Temperature
ConjugateAlexa Fluor 647
For Use With (Equipment)Flow Cytometer
Unit Size120 reactions
Catalog Number
Unit Size
120 reactions
Conjugate
APC (Allophycocyanin)
Excitation/Emission
650/660
Color
Red
Price (USD)
Price: 227.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionAnnexin V APC Ready Flow Conjugate
Kit Contents2 dropper bottles, store at room temperature
Product TypeReady Flow Conjugate
Flow Cytometer Laser LinesRed
Excitation/Emission650/660
ColorRed
Shipping ConditionRoom Temperature
ConjugateAPC (Allophycocyanin)
For Use With (Equipment)Flow Cytometer
Unit Size120 reactions
Showing 4 of 4
Catalog NumberSpecificationsUnit SizeConjugateExcitation/EmissionColorPrice (USD)AvailabilityQuantity
R37177Full specifications
120 reactionsPacific Blue410/455Blue
Price: 227.00
Your Price:
-
DescriptionAnnexin V Pacific Blue Ready Flow Conjugate
Kit Contents2 dropper bottles, store at room temperature
Product TypeReady Flow Conjugate
Flow Cytometer Laser LinesViolet
Excitation/Emission410/455
ColorBlue
Shipping ConditionRoom Temperature
ConjugatePacific Blue
For Use With (Equipment)Flow Cytometer
Unit Size120 reactions
R37174Full specifications
120 reactionsAlexa Fluor 488495/519Green
Price: 227.00
Your Price:
-
DescriptionAnnexin V Alexa Fluor 488 Ready Flow Conjugate
Kit Contents2 dropper bottles, store at room temperature
Product TypeReady Flow Conjugate
Flow Cytometer Laser LinesBlue
Excitation/Emission495/519
ColorGreen
Shipping ConditionRoom Temperature
ConjugateAlexa Fluor 488
For Use With (Equipment)Flow Cytometer
Unit Size120 reactions
R37175Full specifications
120 reactionsAlexa Fluor 647650/665Far-Red
Price: 319.00
Your Price:
-
DescriptionAnnexin V Alexa Fluor 647 Ready Flow Conjugate
Kit Contents2 dropper bottles, store at room temperature
Product TypeReady Flow Conjugate
Flow Cytometer Laser LinesRed
Excitation/Emission650/665
ColorFar-Red
Shipping ConditionRoom Temperature
ConjugateAlexa Fluor 647
For Use With (Equipment)Flow Cytometer
Unit Size120 reactions
R37176Full specifications
120 reactionsAPC (Allophycocyanin)650/660Red
Price: 227.00
Your Price:
-
DescriptionAnnexin V APC Ready Flow Conjugate
Kit Contents2 dropper bottles, store at room temperature
Product TypeReady Flow Conjugate
Flow Cytometer Laser LinesRed
Excitation/Emission650/660
ColorRed
Shipping ConditionRoom Temperature
ConjugateAPC (Allophycocyanin)
For Use With (Equipment)Flow Cytometer
Unit Size120 reactions
Showing 4 of 4
Annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, an indicator of intermediate stages of apoptosis. The difference in fluorescence intensity between apoptotic and nonapoptotic cells stained with our fluorescent annexin V conjugates, as measured by flow cytometry, is typically about 100-fold.

The Annexin V Ready Flow conjugates are provided as ready-to-use, direct-to-sample apoptotic cell stains. Simply add one drop from the dropper bottle directly into 1 x 106 cells in 5 mM Ca2+-containing cell culture medium or annexin binding buffer (Cat. Nos. V13246 or BMS500BB), incubate, and analyze. The traditional annexin staining protocol can be used as an alternative method by replacing the addition of 5 μL annexin conjugate with the addition of 1 drop from the Ready Flow dropper bottle.

The Annexin V Alexa Fluor 488 conjugate is excitable at 495 nm with an emission peak at 519 nm.
The Annexin V Alexa Fluor 647 conjugate is excitable at 650 nm with an emission peak at 665 nm.
The Annexin V APC conjugate is excitable at 650 nm with an emission peak at 660 nm.
The Annexin V Pacific Blue conjugate is excitable at 410 nm with an emission peak at 455 nm.
Annexin V staining to detect apoptotic cells can only be done on live cells and tissue. If samples are to be fixed post-staining, there are specific conditions required to achieve transient retention of signal. These include use of an alcohol-free, aldehyde-based fixation method, use of buffers containing Ca2+ and avoidance of surfactants/detergents. For your convenience, we also offer a concentrated annexin-binding buffer that facilitates the binding of annexin V to phosphatidylserine in apoptosis assays.

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Store at room temperature.