Dermatophyte Test Medium (DTM) (Dehydrated)

Facilitate selective and differential isolation of pathogenic fungi (dermatophytes) from cutaneous sources using Thermo Scientific™ Remel™ Dermatophyte Test Medium (DTM) (Dehydrated). This medium was formulated by Taplin et al. for use in locations where specialized training and microscopic examination are not available¹. A pH indicator and three antimicrobial agents are added to the agar to provide a differential and selective medium for isolation of dermatophytes belonging to the genera Microsporum, Trichophyton, or Epidermophyton.

Dermatophytes are fungi that possess keratinolytic properties that enable them to invade skin, nails and hair². The infections caused by these organisms are commonly referred to as ringworm and are classified by the Latin word tinea followed by the area of the body infected³.

Dermatophyte test medium (DTM) is recommended for selective isolation of pathogenic fungi from cutaneous sources.

• Selective: Cycloheximide, chloramphenicol, and gentamycin inhibit most saprophytic fungi and many gram-positive and gram-negative bacteria, including some Pseudomonas
• Ease of identification: Color change of phenol red indicator from yellow to red

DTM is primarily used as a screening test for detection of dermatophytes. This medium contains soy peptone that supplies the nitrogen and carbon necessary for the growth of microorganisms. Dextrose acts as an energy source. Phenol red is a pH indicator which detects alkaline metabolites produced by dermatophytes, resulting in a color change from yellow to red. Chloramphenicol and gentamycin are broad-spectrum antimicrobials which inhibit a wide range of gram-positive and gram-negative bacteria. Cycloheximide, an antifungal agent, is added to inhibit saprophytic fungi.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.