Remel™ KF Strep Agar

Selectively isolate and enumerate fecal streptococci using Thermo Scientific™ Remel™ KF Strep Agar (Dehydrated). The medium is supplemented with triphenyltetrazolium chloride (TTC). In 1961, Kenner formulated KF Strep Agar to detect fecal streptococci in polluted surface waters¹. This medium is recommended to detect and enumerate enterococci in water, foods, and dairy products in Compendium of Methods for the Microbiological Examination of Foods, Standard Methods for the Examination of Water and Wastewater, and Standard Methods for the Examination of Dairy Products².

Since enterococci are host specific and  survive only a short interval outside their natural habitats, their detection in surface waters may help in identifying the source of pollution³. Enterococci survive in microenvironments unfavorable to coliforms⁴ so are used as an index of sanitary quality in the dairy industry.

Use KF Strep Agar for detection and enumeration of enterococci in water, foods, and dairy products.

• Recommended to detect and enumerate enterococci in water, foods, and dairy products in Compendium of Methods for the Microbiological Examination of Foods, Standard Methods for the Examination of Water and Wastewater, and Standard Methods for the Examination of Dairy Products^2-4
• Selective medium - sodium azide makes the medium selective

This medium contains casein and meat peptones which provide amino acids, peptides, and nitrogenous compounds that are necessary for the bacterial growth. Yeast extract enhances the growth as it is a source of B-complex vitamins. Sodium chloride maintains osmotic equilibrium. Maltose and lactose are fermentable carbohydrates present in the medium. Brom cresol purple is an indicator dye. Triphenyltetrazolium chloride (TTC) is reduced by enterococci to its formazan derivatives resulting in deep red colored colonies. Agar is a solidifying agent.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.