Middlebrook 7H9 Broth (Dehydrated)

Isolate and cultivate Mycobacterium spp. using Thermo Scientific™ Remel™ Middlebrook 7H9 Broth (Dehydrated). In 1958, Middlebrook and Cohn were the first to formulate this medium for the cultivation of mycobacteria¹. This medium is found useful in the transfer of cultures to be tested with biochemical media².

Isolate and cultivate Mycobacterium spp. using Middlebrook 7H9 Broth.

• Ease of Handling: Ease of transfer of cultures to be tested with biochemical media.
• Allows luxuriant growth of Mycobacterium

This medium is used for qualitative procedures. This medium contains inorganic salts which are essential for the growth of mycobacteria. Ammonium sulphate acts as a source of nitrogen. Biotin plays an important role in carboxylation and decarboxylation reactions, while pyridoxin is a precursor of enzymatic activity. The addition of ADC Enrichment provides a carbon source of energy, and catalase minimizes toxic effects.

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