The FreeStyle™ 293-F cell line is derived from the 293 cell line and is intended for use with the FreeStyle™ MAX 293 Expression System or FreeStyle™ 293 Expression System. FreeStyle™ 293-F cells are adapted to suspension culture in FreeStyle™ 293 Expression Medium. Frozen cells are supplied in and may be thawed directly into FreeStyle™ 293 Expression Medium. The FreeStyle™ 293-F cell line is supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in 90% FreeStyle™ 293 Expression Medium and 10% DMSO. Characteristics of FreeStyle™ 293-F cells:
• Prepared from low-passage Master Cell Bank cultures derived from parental 293-F cells that were re-cloned by limiting dilution. The 293 clonal derived cultures are maintained in serum-free conditions for only 30 to 35 total passages. • Adapted to high-density, serum-free, suspension growth and may be maintained in FreeStyle™ 293 Expression Medium. • Demonstrate high transfection efficiencies with FreeStyle™ MAX reagent. • Suspension cultures may be transfected in FreeStyle™ 293 Expression Medium without the need to change media. • Permits transfection of cells at large volumes.
FreeStyle™ 293-F Cells
FreeStyle™ 293 Expression Medium
For Use With (Application)
Protein Expression and Transfection
Contents & Storage
1 vial of 1 x 107 cells. Store in liquid nitrogen.