Search

  • Order Status
  • Quick Order
  • Promos
HisG Epitope Tag Monoclonal Antibody, AP - FAQs

HisG Epitope Tag Monoclonal Antibody, AP - FAQs

View additional product information for HisG Epitope Tag Monoclonal Antibody, AP - FAQs (R94225)

2 product FAQs found

How do you typically detect expression of a recombinant fusion protein?

Western blot analysis is typically used to detect the expressed protein. We sell several antibodies against various epitopes, such as Xpress, HisG, V5, or C-terminal 6xHis. Additionally, His-tagged proteins can be purified using our ProBond Purification System via affinity purification.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I use the anti-HisG antibody for western detection of the His tag in Gateway pT-REx DEST31?

The Gateway pT-REx DEST31 vector contains an N-terminal 6xHis tag (the 6XHis in this vector is not followed by Glycine (G) or -COOH). Hence, it cannot be detected using anti-HisG or anti-His (C-terminal) antibodies. Instead, we recommend using an anti-6xHis antibody (Cat. No. 372900).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.