Thermo Scientific™

Lab Vision™ Estrogen Receptor (SP1), Rabbit Monoclonal Antibody

 Related applications:
Anatomical Pathology

Ensure accurate, reproducible results in immunohistochemistry and western blotting experiments with Thermo Scientific™ Estrogen Receptor (SP1), Rabbit Monoclonal Antibody, part of a comprehensive antibody portfolio. Studies show rabbit monoclonal antibodies are more sensitive and specific than other available markers.

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Description

Recognizes a protein of 67 kDa, which is identified as estrogen receptor (ER) alpha. The ER gene consists of more than 140kb of genomic DNA divided into 8 exons, and is translated into a protein with six functionally discrete domains, labeled A through F. This antibody strongly stains the nucleus of epithelial cells in breast carcinomas. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.

Host Species: Rabbit

Clone: SP1

Isotype: IgG

Species Reactivity: Human, Mouse and Hamster. Others not tested.

Epitope: C-terminus

Immunogen: Synthetic peptide derived from C-terminal of human estrogen receptor

Molecular Weight: 67 kDa

Positive Control: Breast CA, MCF-7 cells

Cellular Localization: Nuclear

Recommended for:

  • Immunohistochemistry (Formalin/paraffin)
  • Western Blotting
  • Immunofluorescence (Not verified)
  • Flow Cytometery (Not verified)

General References:

1. Huang Z., et al. Novel Rabbit Monoclonal Antibody to Estrogen Receptor (Clone SP1): No Heat Pretreatment But Effective on Paraffin-embedded Tissue. (In press) Applied Immunohistochemistry & Molecular Morphology, USA 2004

2. Cano G., et al. Estimation of Hormone Receptor Status in Fine-Needle Aspirates and Paraffin-embedded Sections from Breast Cancer Using the Novel Rabbit Monoclonal antibodies SP1 and SP2. Diagn Cytopathol, 2003 Oct 29(4): 207 -11



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