Human Melanocyte Growth Supplement-2 (HMGS-2), PMA-free

Human Melanocyte Growth Supplement-2 (HMGS-2), PMA-free, is a sterile, concentrated (100X) solution intended for use with Medium 254 or Medium 254CF to culture human epidermal melanocytes. Each 5-mL bottle of HMGS-2 contains all of the growth factors, hormones, and tissue extracts necessary for the culture of human epidermal melanocytes and is the correct amount of supplement for a 500-mL bottle of medium.

When a 500-mL bottle of medium is supplemented with HMGS-2, the final concentrations of the components in the supplemented medium are:

• Fetal bovine serum: 0.5%
• Basic fibroblast growth factor: 3 ng/mL
• Bovine pituitary extract: 0.2%
• Heparin: 3 μg/mL
• Hydrocortisone: 0.18 μg/mL
• Recombinant human insulin-like growth factor-I: 0.01 μg/mL
• Transferrin: 5 μg/mL
• Endothelin-1: 10 nM