SYTOX™ AADvanced™ Dead Cell Stain Kit
SYTOX™ AADvanced™ Dead Cell Stain Kit
Invitrogen™

SYTOX™ AADvanced™ Dead Cell Stain Kit

SYTOX™ AADvanced™ Dead Cell Stain (S10274, S10349) is a new high-affinity nucleic acid stain for the detection of dead cellsRead more
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Catalog NumberQuantity
S10274500 Reactions
S10349100 Reactions
Catalog number S10274
Price (USD)
307.00
Each
Add to cart
Quantity:
500 Reactions
Price (USD)
307.00
Each
Add to cart
SYTOX™ AADvanced™ Dead Cell Stain (S10274, S10349) is a new high-affinity nucleic acid stain for the detection of dead cells and analysis of cell cycle using the common 488 nm blue laser in flow cytometry. The dye is spectrally similar to 7-AAD but with rapid kinetics of uptake and relatively low CVs. SYTOX™ AADvanced™ Dead Cell Stain penetrates cells more efficiently than 7-AAD providing better separation of live and dead cells. SYTOX™ AADvanced™ Dead Cell Stain can also be used with fixed cells for DNA content analysis when paired with RNAse treatment. SYTOX™ AADvanced™ Dead Cell Stain Kits contain a vial(s) of dried dye and anhydrous DMSO providing stable shelf life.

View a selection guide for all nonfixable viability dyes for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell PermeabilityImpermeant
DescriptionSYTOX™ AADvanced™ Dead Cell Stain Kit
Detection MethodFluorescence
Dye TypeSYTOX™ AADvanced™
FormSolid
FormatTube(s)
Quantity500 Reactions
Shipping ConditionRoom Temperature
SolubilityDMSO (Dimethylsulfoxide)
Sub Cellular LocalizationNucleus, Nucleic Acids
ColorRed
Emission647
Excitation Wavelength Range546 nm
For Use With (Application)Viability Assay
For Use With (Equipment)Flow Cytometer
Product LineMolecular Probes, SYTOX
Product TypeDead Cell Stain Kit
Unit SizeEach
Contents & Storage
Contains 5 vials of SYTOX™ AADvanced™ dead cell stain and 500 μL dimethylsulfoxide (DMSO).

Store at ≤-20°C and protect from light.

Frequently asked questions (FAQs)

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am using SYTOX AAdvanced as a dead cell stain, but all of my cells are labeling even though I am certain that they are supposed to be alive. These are adherent cells that I have trypsinized. Why am I getting false-dead signals?

SYTOX AAdvanced labels only dead cells because it is a cell impermeant dye. The dye can only enter cells that have a compromised plasma membrane. Trypsinization may cause temporary disruption of the plasma membrane, sufficient to allow staining with a cell impermeant dye. You can reduce the “false-dead” problem by either reducing the amount of trypsin and/or reduce the incubation time for trypsinization or use a gentler dissociation reagent such as TrypLE Express, TrypLESelect reagents, or Versene. After trypsinization, wash well, and if possible, allow a recovery time in normal culture media before staining with any of the SYTOX dyes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

My cell cycle data show a single peak, not a proper cell cycle profile. How can I fix this?

There are several factors that contribute to the quality of the cell cycle profile. Cell number, dye concentration, incubation temperature, incubation time, flow rate (on a traditional flow cytometer utilizing hydrodynamic focusing), total number of cells acquired, elimination of dead cells, and removal of aggregates from data analysis should all be considered when analyzing the cell cycle.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.