Invitrogen™
SYTOX™ AADvanced™ Dead Cell Stain Kit
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Description
SYTOX™ AADvanced™ Dead Cell Stain (S10274, S10349) is a new high-affinity nucleic acid stain for the detection of dead cells and analysis of cell cycle using the common 488 nm blue laser in flow cytometry. The dye is spectrally similar to 7-AAD but with rapid kinetics of uptake and relatively low CVs. SYTOX™ AADvanced™ Dead Cell Stain penetrates cells more efficiently than 7-AAD providing better separation of live and dead cells. SYTOX™ AADvanced™ Dead Cell Stain can also be used with fixed cells for DNA content analysis when paired with RNAse treatment. SYTOX™ AADvanced™ Dead Cell Stain Kits contain a vial(s) of dried dye and anhydrous DMSO providing stable shelf life.View a selection guide for all nonfixable viability dyes for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.
Figures
Multiparameter apoptosis assay.
Caspase activity detection in Jurkat cells using the CellEvent® Caspase-3/7 Green Flow Cytometry Assay Kit on the Attune® Flow Cytometer.
A mixed population of Jurkat cells were stained with SYTOX® AADvanced™ Dead Cell Stain Kit (S10274) and analyzed by flow cytometry
Specifications
| For Use With (Equipment): | Flow Cytometer |
|---|---|
| Cell Permeability: | Cell-Impermeant |
| Sub-Cellular Localization: | Nucleic Acids, Nucleus |
| Detection Method: | Fluorescent |
| Excitation⁄Emission (nm): | 546⁄647 |
| Flow Cytometer Laser Lines: | 488, 532, 561 |
| Form: | Solid |
| Format: | Tube(s) |
| Label or Dye: | SYTOX® AADvanced™ |
| Number of Reactions: | 500 |
| Product Line: | Molecular Probes®, SYTOX® |
| Product Size: | 1 kit |
| Solubility: | DMSO (Dimethylsulfoxide) |
| Shipping Condition: | Room Temperature |
Contents & storage
Contains 5 vials of SYTOX® AADvanced™ dead cell stain and 500 µL dimethylsulfoxide (DMSO).
Store at ≤-20°C and protected from light.
Store at ≤-20°C and protected from light.
