SYTO™ 40 Blue Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

Citas y referencias (6)

Invitrogen™

SYTO™ 40 Blue Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

La tinción de ácido nucleico azul fluorescente permeable en células SYTO 40 exhibe fluorescencia azul brillante al enlazarse con losMás información

Número de catálogo	Cantidad
S11351	250 μL

Número de catálogo S11351

Precio (MXN)

Cantidad:

250 μL

La tinción de ácido nucleico azul fluorescente permeable en células SYTO 40 exhibe fluorescencia azul brillante al enlazarse con los ácidos nucleicos. Debido a que el patrón de tinción de SYTO en las células vivas puede variar entre los diferentes tipos de células, ofrecemos el kit de muestreo para tinción de ácido nucleico azul fluorescente SYTO (S-11350) para permitir a los investigadores encontrar el colorante más adecuado para su sistema.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

ColorRojo, azul

DescripciónTinción de ácido nucleico SYTO™ 40 Blue Fluorescent: solución de 5 mM en DMSO

Método de detecciónFluorescente

Tipo de colorantePermeabilidad celular

Emisión441 nm

Intervalo de longitud de onda de excitación420 nm

Para utilizar con (equipo)Microscopio de fluorescencia

Línea de productosSYTO

Cantidad250 μL

Condiciones de envíoTemperatura ambiente

Volumen (métrico)250 μl

Tipo de etiquetaFluorescente

Tipo de productoTinción de ácidos nucleicos

SubCellular LocalizationÁcidos nucleicos

Unit SizeEach

Contenido y almacenamiento

Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (6)

Citations & References

Abstract

Nucleic acid binding agents exert local toxic effects on neurites via a non-nuclear mechanism.

Authors:Pin S, Chen H, Lein PJ, Wang MM

Journal:J Neurochem

PubMed ID:16441515

'The mechanism by which drugs that target nucleic acids cause neurotoxicity is not well described. We characterized the neurotoxicity of Hoechst 33342 (bis-benzimide), a common cell permeable nuclear dye, in primary neuronal cultures. The mechanism of cell death was not apoptotic, as death is rapid, not accompanied by typical nuclear ... More

The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis-2-Decenoic Acid.

Authors:Amari DT, Marques CN, Davies DG,

Journal:

PubMed ID:23935049

'In the present study, we report the identification of a putative enoyl-coenzyme A (CoA) hydratase/isomerase that is required for synthesis of the biofilm dispersion autoinducer cis-2-decenoic acid in the human pathogen Pseudomonas aeruginosa. The protein is encoded by PA14_54640 (PA0745), named dspI for dispersion inducer. The gene sequence for this ... More

Specific and rapid enumeration of viable but nonculturable and viable-culturable gram-negative bacteria by using flow cytometry.

Authors:Khan MM, Pyle BH, Camper AK,

Journal:Appl Environ Microbiol

PubMed ID:20543046

An issue of critical concern in microbiology is the ability to detect viable but nonculturable (VBNC) and viable-culturable (VC) cells by methods other than existing approaches. Culture methods are selective and underestimate the real population, and other options (direct viable count and the double-staining method using epifluorescence microscopy and inhibitory ... More

Close relation of arterial ICC-like cells to the contractile phenotype of vascular smooth muscle cell.

Authors:Pucovský V, Harhun MI, Povstyan OV, Gordienko DV, Moss RF, Bolton TB,

Journal:J Cell Mol Med

PubMed ID:17760838

This work aimed to establish the lineage of cells similar to the interstitial cells of Cajal (ICC), the arterial ICC-like (AIL) cells, which have recently been described in resistance arteries, and to study their location in the artery wall. Segments of guinea-pig mesenteric arteries and single AIL cells freshly isolated ... More

Subcutaneous Injections of Nanofat Adipose-derived Stem Cell Grafting in Facial Rejuvenation.

Authors:

Journal:Plast Reconstr Surg Glob Open

PubMed ID:32095390

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