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Streptavidin, Alexa Fluor® 790 Conjugate

Catalog number: S11378

Molecular Probes™  Related applications: Protein Assays and Analysis | Secondary Detection

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Description

Alexa Fluor® 790 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Alexa Fluor® dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor® 790 Streptavidin Conjugates:

Alexa Fluor® 790 streptavidin conjugate has Ex/Em maxima of ~(782/805)
Bright, photostable fluorescence
High solubility in aqueous solutions
Available in multiple colors
Ideal for western blots

Properties of Alexa Fluor® Dyes
Alexa Fluor® dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

Learn more about Alexa Fluor® Dyes

Find out about other Labeled Streptavidin Conjugates

Read Avidin and Streptavidin Conjugates—Section 7.6 in the Molecular Probes Handbook

Specifications

Label or Dye: Alexa Fluor® 790
Product Size: 1 mg
Shipping Condition: Room Temperature

Contents & storage

Store at ≤-20°C

Protect from light

Desiccate

Documents

Manuals & protocols

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