Invitrogen™

SiteClick™ Biotin Antibody Labeling Kit

Catalog number:  S20033

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Description

Unlike the conventional amine-thiol crosslinker labeling method, the SiteClick Biotin Antibody Labeling Kit specifically attaches the label to the heavy chains of an IgG antibody, ensuring that the antigen-binding domains remain available for binding to the antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. In addition, no harsh reduction steps are required, and the labeling is consistent and reproducible.

Features of the SiteClickBiotin Antibody Labeling Kit:
• Contains everything required to label 100–250 µg of IgG antibody
• Easy-to-follow step-by-step protocol
• Highly efficient, site-specific, reproducible labeling chemistry results in high quality antibody conjugate

Learn more about SiteClick labeling technology ›
Learn more about streptavidin detection ›
Learn more about protein and antibody labeling › (We offer a wide selection of antibody and protein labeling kits.)

Custom SiteClick Antibody Labeling Service
If you have an antibody that is considered "difficult to label" or has lost activity after labeling using a conventional method, please contact our custom service representatives to determine whether the SiteClick Antibody Labeling Service would be right for your antibody. We offer complete custom SiteClick antibody labeling services with the option of multiple detection molecules including biotin, Alexa Fluor dyes, Qdot fluorophores, R-PE, chelates for PET imaging, and many others.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Labeling Scale: 100-250 µg
Labeling Target: Antibodies (General)
Product Line: SiteClick™
Product Size: 1 kit
Shipping Condition: Room Temperature

Contents & storage

Store in refrigerator (2–8°C), desiccate, protect from light, and do not freeze.

Documents

Manuals & protocols