Invitrogen™
Streptavidin, Alexa Fluor™ 610—R-Phycoerythrin Conjugate (Alexa Fluor™ 610—R-Phycoerythrin Streptavidin)
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Description
The streptavidin, Alexa Fluor™ 610-R-phycoerythrin streptavidin tandem conjugate has excitation and emission maxima at ~496,565/627 and is widely used to detect biotinylated probes by flow cytometry. Not only are the Alexa Fluor™ 610 conjugates of R-PE are more fluorescent than the commercially available Texas Red™ conjugates of R-PE, the fluorescent emission of Alexa Fluor™ 610-R-PE tandem conjugates is shifted to somewhat longer wavelengths than is the emission of Texas Red™-R-PE conjjgates, resulting in better separation from the emission of R-PE.View a selection guide for all Alexa Fluor® streptavidin and avidin conjugates.
For Research Use Only. Not for use in diagnostic procedures.
Figures
Simultaneous detection of three cell surface markers using an Alexa Fluor® 610–R-phycoerythrin tandem conjugate, Alexa Fluor® 488 dye and R-phycoerythrin labels.
Dual color flow cytometry dot plot showing mouse anti human CD8 RPE vs. mouse anti human CD4 biotin followed by streptavidin RPE-Alexa Fluor® 610.
Simultaneous detection of three cell surface markers using an Alexa Fluor® 610–R-phycoerythrin tandem conjugate, Alexa Fluor® 488 dye and R-phycoerythrin labels.
Specifications
| Detection Method: | Fluorescent |
|---|---|
| Flow Cytometer Laser Lines: | 488 or 561 |
| Label or Dye: | PE-Alexa Fluor® 610 |
| Concentration: | 1 mg⁄ml |
| Excitation⁄Emission (nm): | 488 or 561⁄630 |
| For Use With (Equipment): | Blot Analysis, Flow Cytometry, Histochemical Applications, Immunoassays |
| Form: | Solution |
| Product Line: | Alexa Fluor™ |
| Product Size: | 100 µL |
| Shipping Condition: | Wet Ice |
Contents & storage
Contains 1 vial of streptavidin R-PE-Alexa Fluor® 610 conjugate (1 mg/mL).
Store in refrigerator (2–8°C) and protect from light.
Store in refrigerator (2–8°C) and protect from light.
