Streptavidin, Alexa Fluor™ 610—R-Phycoerythrin Conjugate (Alexa Fluor™ 610—R-Phycoerythrin Streptavidin)
Invitrogen™

Streptavidin, Alexa Fluor™ 610—R-Phycoerythrin Conjugate (Alexa Fluor™ 610—R-Phycoerythrin Streptavidin)

Estreptavidina, conjugado Alexa Fluor™ 610—R-ficoeritrina (estreptavidina Alexa Fluor™ 610—R-ficoeritrina)
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Número de catálogoCantidad
S20982
también denominado S-20982
100 μl
Número de catálogo S20982
también denominado S-20982
Precio (MXN)
-
Cantidad:
100 μl

The streptavidin, Alexa Fluor™ 610-R-phycoerythrin streptavidin tandem conjugate has excitation and emission maxima at ∼496,565/627 and is widely used to detect biotinylated probes by flow cytometry. Not only are the Alexa Fluor™ 610 conjugates of R-PE are more fluorescent than the commercially available Texas Red™ conjugates of R-PE, the fluorescent emission of Alexa Fluor™ 610-R-PE tandem conjugates is shifted to somewhat longer wavelengths than is the emission of Texas Red™-R-PE conjjgates, resulting in better separation from the emission of R-PE.

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Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Concentración1 mg/mL
Método de detecciónFluorescente
Excitación/emisión488 o 561/630
Líneas láser del citómetro de flujo488 o 561
Para utilizar con (equipo)Citometría de flujo, inmunoensayos, aplicaciones histoquímicas, análisis de blot
Etiqueta o tintePE-Alexa Fluor 610
Tipo de productoConjugado de estreptavidina (Fluorescente)
Cantidad100 μl
Condiciones de envíoHielo húmedo
ConjugadoPE-Alexa Fluor 610
FormularioSolución
Línea de productosAlexa Fluor
Unit SizeEach
Contenido y almacenamiento
Contiene un 1 vial de estreptavidina, conjugado R-PE-Alexa Fluor™ 610 (1 mg/ml).

Almacenar en el refrigerador (2–8 °C) y proteger de la luz.

Preguntas frecuentes

Can I use my tandem dye antibody (e.g., Alexa Fluor 610-R-phycoerythrin) for microscopy?

Tandem dyes are not recommended for imaging applications. Both R-PE or APC tend to photobleach quickly and cannot be archived. Any unfolding of the phycobiliprotein would result in loss of fluorescence. They work great in flow cytometry applications.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (2)

Citations & References
Abstract
Authors:
Journal:
PubMed ID:18258751
Nucleoside salvage pathway kinases regulate hematopoiesis by linking nucleotide metabolism with replication stress.
Authors:Austin WR, Armijo AL, Campbell DO, Singh AS, Hsieh T, Nathanson D, Herschman HR, Phelps ME, Witte ON, Czernin J, Radu CG,
Journal:J Exp Med
PubMed ID:23148236
'Nucleotide deficiency causes replication stress (RS) and DNA damage in dividing cells. How nucleotide metabolism is regulated in vivo to prevent these deleterious effects remains unknown. In this study, we investigate a functional link between nucleotide deficiency, RS, and the nucleoside salvage pathway (NSP) enzymes deoxycytidine kinase (dCK) and thymidine ... More