SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor™ 680

SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor™ 680 - FAQs

View additional product information for SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor™ 680 - FAQs (S30045)

4 product FAQs found

What amount of conjugated antibody should I inject to image tumors?

A recommended starting dosage is 50 µg. You will need to determine the optimal dosage for your experimental model.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What size needle should I use for small animal in vivo imaging?

We recommend the use of a 28-32 gauge tuberculin or insulin syringe (0.3 or 1.0 mL volume) with a fixed (non-removable) needle.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the optimal and maximal volumes of reagent that can be injected into a mouse?

The volume of reagent that can be injected varies according to the route of administration. The following numbers are general guidelines for a 25 gram animal: Intravenous (IV)- 50-125 µl (recommended)- 200µl (maximum) ; Intraperitoneal (IP) 500µl (recommended) -2ml (maximum) ; Subcutaneous(SC) 100-250 µl (recommended)- 1ml (maximum).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of mice are the best for in vivo imaging?

Due to light scattering caused by fur, hairless mice such as athymic nude (nu/nu) mice are recommended for in vivo imaging. If this is not an option, the hair covering the area to be imaged should be removed using clippers or a chemical depilatory such as Nair depilatory.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.