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View additional product information for SYTO™ Green Fluorescent Nucleic Acid Stains - FAQs (S32703, S7575, S7574, S7573, S7572, S7578, S7556, S34854, S7576, S34855, S7559)
2 product FAQs found
The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:
1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid.
4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).
SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
The reagent for exosome isolation from serum can be used with serum from any species in addition to human. When working with small volumes of non-human samples (e.g., mouse) or when it is desired to maximize the recovery of exosomes, we recommend following the standard protocol except for spinning the samples (after incubation with the reagent) to precipitate the exosomes at 4 degrees C.