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View additional product information for SYTO® 10 Green Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO - FAQs (S32704)
2 product FAQs found
The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:
1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid.
4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).
SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Plasma is a more challenging type of sample compared to serum. It has rather high levels of clotting factors. The current serum reagent will work on plasma, and it will precipitate all exosomes. However, the preparation will have some contaminating proteins and microvesicles, which will work for some projects, but not be acceptable for others. We recommend using our specifically optimized kits for recovery of exosomes from blood plasma (Cat. No. 4484450), as well as urine (Cat. No. 4484452), or other body fluids (Cat. No. 4484453).