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Citations & References (5)
Invitrogen™
Streptavidin, β-galactosidase conjugate
Streptavidin, β-galactosidase conjugate
| Catalog Number | Quantity |
|---|---|
| S931 also known as S-931 | 1 mg |
Catalog number S931
also known as S-931
Price (USD)
432.00
Each
Quantity:
1 mg
Price (USD)
432.00
Each
The streptavidin, ß-galactosidase conjugate can be used to detect biotin in a signal amplification scheme in conjunction with chromogenic substrates or fluorogenic substrates.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeStreptavidin Conjugate (fluorescent)
Quantity1 mg
Shipping ConditionWet Ice
Conjugatebeta-Galactosidase
FormSolid
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C).
Citations & References (5)
Citations & References
Abstract
Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays.
Journal:J Immunol Methods
PubMed ID:2558138
'A novel signal amplification method, catalyzed reporter deposition (CARD), and its application to immunoassays is described. The method involves utilizing an analyte-dependent reporter enzyme (ADRE) to catalyze the deposition of additional reporter on the surface in a solid-phase immunoassay. In the examples described, deposition of reporter is facilitated by using
Fluorescence probe studies of the state of tropomyosin in reconstituted muscle thin filaments.
Journal:Biochemistry
PubMed ID:3663633
'The monomer fluorescence of N-(1-pyrenyl)maleimide-labeled tropomyosin bound to F-actin (PTm-actin) increases when myosin subfragment 1 (S1) binds to actin and is half complete when only approximately 1 S1 is bound to 7 actin subunits [Ishii, Y., & Lehrer, S. S. (1985) Biochemistry 24, 6631-6638]. Similar studies of the binding of
Fluorescence sandwich enzyme-linked immunosorbent assay for detecting human interleukin-2 receptors.
Journal:J Immunol Methods
PubMed ID:2453583
A very sensitive fluorescence sandwich enzyme-linked immunosorbent assay (FS-ELISA) for the detection of soluble and cell-associated human interleukin-2 receptors (IL-2R) has been developed. For use in this assay system, two anti-human IL-2R monoclonal antibodies, H 48 and biotinylated HA 26, were selected from six monoclonal antibodies directed against different epitopes
Measurement of antigen-specific mouse IgE by a fluorometric reverse (IgE-capture) ELISA.
Journal:J Immunol Methods
PubMed ID:2783445
A reverse, or IgE-capture, enzyme-linked immunosorbent assay (ELISA) for measuring ovalbumin-specific IgE antibody in the serum of immunized mice has been developed. Microplate wells were first coated with a commercial anti-mouse IgE rat monoclonal antibody, and then incubated with two-fold serial dilutions of test sera with 10% normal mouse serum
TNF-alpha pretreatment prevents subsequent activation of cultured brain cells with TNF-alpha and hypoxia via ceramide.
Journal:Am J Physiol
PubMed ID:10329967
We have developed a cellular model in which cultured astrocytes and brain capillary endothelial cells preconditioned with tumor necrosis factor-alpha (TNF-alpha) fail to upregulate intercellular adhesion molecule-1 (ICAM-1) protein (80% inhibition) and mRNA (30% inhibition) when challenged with TNF-alpha or exposed to hypoxia. Inasmuch as ceramide is known to mediate