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Invitrogen™

Streptavidin-Horseradish Peroxidase (HRP) Conjugate

Catalog Number	Quantity
SA10001	50 μL

Catalog number SA10001

Price (USD)

349.00

Quantity:

50 μL

Price (USD)

349.00

Streptavidin-HRP conjugate is typically used in the immunodetection of biotinylated proteins. This conjugate is suitable for use in immunoblotting, ELISA, immunomicroscopy. The amount of conjugate provided is sufficient for 25 western blots using 10 ml of working solution per reaction or 25 immunostaining reactions using 1 ml of working solution per reaction as appropriate.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration0.75 to 1.2 mg/mL

Label or DyeHRP (Horseradish Peroxidase)

Product TypeStreptavidin Conjugate (fluorescent)

Quantity50 μL

ConjugateHRP

FormLiquid

Product LineInvitrogen

Unit SizeEach

Contents & Storage

Store in refrigerator (2—8°C).

Frequently asked questions (FAQs)

Should I block my western blot membrane before incubating with Streptavidin-Horseradish Peroxidase (HRP) Conjugate (Cat. No. SA10001)?

Yes, you should block the western blot membrane before incubating with Streptavidin-HRP Conjugate. Blocking is essential to reduce non-specific binding and background signal.
We recommend incubating the membrane in 10 mL blocking solution. Gently agitate using a rocker platform for 1 hour at room temperature and then proceed to washing the membrane (Step 5 of the protocol on page 5 of the user manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/streptavidin_conjugates_man.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.