Invitrogen™

ELISA TMB Stabilized Chromogen

Catalog number:  SB02

Related applications:

Protein Assays and Analysis

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Description

ELISA TMB Stabilized Chromogen is a ready-to-use one-component horseradish peroxidase substrate (HRP) for ELISA plate applications. It can be used in both kinetic assays (optical density monitored over several time points) and in endpoint assays. The solution is colorless or pale blue prior to use. In the presence of peroxidase, the solution develops a blue reaction product. The optical density of the solution is measured at two wavelengths: 492 nm and 650 nm.

When color formation is monitored at an endpoint, an equal volume of an acidic stop solution should be added to the wells. Addition of a stop solution results in the rapid formation of a yellow end product. The optical density of this yellow end product is measured at 450 nm.

For a complete kit of ELISA buffers, see ELISA Buffer Kit.
View all available buffers and reagents for ELISA applications.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Colorimetric
Shipping Condition: Wet Ice
Substrate: TMB
Substrate Properties: Chemical Substrate
Substrate Type: HRP (Horseradish Peroxidase) Substrate

Contents & storage

Solution. Store at 2-8°C.