Thermo Scientific™

Oxoid™ ONE Broth-Listeria Supplement

Catalog number: SR0234B
Thermo Scientific™

Oxoid™ ONE Broth-Listeria Supplement

Catalog number: SR0234B

The Oxoid ONE Broth-Listeria is for the selective enrichment broth for Listeria species from food samples in 24 hours.

 
Catalog Number
SR0234B
Unit Size
Each
Yield
Each vial for 2.25L medium
Price (USD)
Full specifications
DescriptionONE Broth-Listeria Supplement
Product TypeSupplement
SpeciesListeria
YieldEach vial for 2.25L medium
Quantity10/Pk.
TypeSupplement
FormatFreeze Dried
Unit SizeEach
Catalog Number
Unit Size
Each
Yield
Each vial for 225mL medium
Price (USD)
Full specifications
DescriptionONE Broth-Listeria Supplement
Product TypeSupplement
SpeciesListeria
YieldEach vial for 225mL medium
Quantity10/Pk.
TypeSupplement
FormatFreeze Dried
Unit SizeEach
Showing 2 of 2
Catalog NumberSpecificationsUnit SizeYieldPrice (USD)
SR0234BFull specifications
EachEach vial for 2.25L mediumRequest A Quote
DescriptionONE Broth-Listeria Supplement
Product TypeSupplement
SpeciesListeria
YieldEach vial for 2.25L medium
Quantity10/Pk.
TypeSupplement
FormatFreeze Dried
Unit SizeEach
SR0234EFull specifications
EachEach vial for 225mL mediumRequest A Quote
DescriptionONE Broth-Listeria Supplement
Product TypeSupplement
SpeciesListeria
YieldEach vial for 225mL medium
Quantity10/Pk.
TypeSupplement
FormatFreeze Dried
Unit SizeEach
Showing 2 of 2
Add to ONE Broth-Salmonella, Part No. CM1091B.

ONE Broth - Listeria: Typical Formulation

  • Peptone: 26g/L; Carbohydrate mix: 6g/L; Salt mix: 10g/L with a final pH of 7.4 ±0.2 at 25°C

Appearance:

  • Dehydrated medium: straw coloured, free-flowing powder
  • Selective supplement: orange/yellow, freeze-dried pellet
  • Prepared medium: dark straw coloured, clear liquid

Note:

Formulation Directions

  • Suspend 22g of ONE Broth-Listeria Base in 500mL (44g/L) of distilled water
  • Mix well and sterilize by autoclaving at 121°C for 15 minutes
  • Cool the medium to below 50°C and aseptically add the contents of one vial of ONE Broth-Listeria Selective Supplement (Mfr. No. SR0234) reconstituted as directed

Testing Protocol

  • Add 25g of food sample to 225mL of ONE Broth-Listeria and stomach for a minimum of 30 seconds to mix the sample
  • Incubate the broth without agitation at 30°C for 24±2 hours
  • Gently agitate the bag then, using a microbiological loop, remove 10μL and inoculate onto a suitable agar medium e.g. Brilliance Listeria Agar (Mfr. No. CM1080, SR0222, and SR0228) and incubate appropriately (37°C for 24-48 hours)

  • Confirm presumptive colonies on the agar plates as Listeria monocytogenes or Listeria spp. by appropriate methods e.g. Gram-stain, catalase, oxidase, Oxoid Listeria Latex Test Kit (Mfr. No. DR1126)
  • Oxoid O.B.I.S. mono (Mfr. No. ID0600), Microbact Listeria 12L (Mfr. No. MB1128)

For Laboratory Use Only

Figures

Frequently asked questions (FAQs)

Citations & References