ELISA-Light™ Immunoassay System with CSPD™ and Sapphire-II™ Substrate/Enhancer Solution

Catalog number: T1023

Applied Biosystems™  Related applications: Protein Assays and Analysis

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Description

The ELISA-Light™ Immunoassay System with CSPD® and Sapphire-II™ Substrate⁄Enhancer Solution is used for ultra-sensitive detection of antigens or antibodies in multiple immunoassay formats employing an alkaline phosphatase enzyme label.

• Heterogeneous assay format increases sensitivity and dynamic range and reduces interference by test compounds in the detection portion of the assay (as compared with homogeneous assay formats)
• Chemiluminescent immunoassay detection allows greater sensitivity and improved dynamic range as compared to radioimmunoassays and immunoassays that employ colorimetric or fluorescent detection
• High sensitivity and wide dynamic range permit sensitive detection with fewer assays, because sample dilution to fit the dynamic range of the assay is unnecessary
• A variety of ready-to-use substrate formulations lets the user precisely optimize signal intensity to the exact requirements of an assay
• The kinetics of the glow-assay provides a "window" during which measurements may be performed, facilitating HTS applications where assay automation is used
• Chemiluminescent immunoassay detection provides versatile applications such as sandwich immunoassays, competitive immunoassays, and DNA probe capture assays
• Non-radioactive immunoassays eliminate concerns over use of radioisotopes

Extend the Limits of Detection
The ELISA-Light™ system offers an excellent combination of high sensitivity and throughput for assays measured in a luminometer. The ELISA-Light™ system significantly extends the limits of detection of most immunoassays previously performed with colorimetric and isotopic methods.

Multiple Substrate⁄Enhancer Formulations
The kit contains reagents optimized for chemiluminescent ELISAs employing alkaline phosphatase labels and is available with a choice of ready-to-use chemiluminescent substrate formulations containing CSPD® or CDP-Star® substrate with Sapphire-II™ or Emerald-II™ luminescence enhancers. Multiple substrate⁄enhancer formulations provide options that let the user optimize specific assays or luminometers. The specific formulation that will provide the highest sensitivity for a specific assay can vary. Therefore, an ELISA sampler kit containing all four substrate⁄enhancer formulations is available to facilitate assay development.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Sensitivity: See product documentation
Detection Method: Chemiluminescent
For Use With (Equipment): Microplate Reader
Former Identifiers: Novex
Gene ID: Non-Gene
Gene Symbol: Non-Gene
Immunoassay Kit Format: Various Other
Label or Dye: AP (Alkaline Phosphatase)
Product Line: ELISA-Light™
Product Size: 1,000 assays
Target (Kit Named): [Generic]

Contents & storage

This kit contains enough reagent for 1000 assays. Store at 4°C.

Documents

Manuals & protocols