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Invitrogen™

Tubulin Tracker™ Green (Oregon Green™ 488 Taxol, bis-acetate)

Catalog number:  T34078

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Description

Tubulin Tracker Green (Oregon Green 488 Taxol, bis-acetate) provides green-fluorescent staining of polymerized tubulin in live cells. Tubulin Tracker Green is an uncharged, non-fluorescent compound that easily passes through the plasma membrane of live cells. Once inside the cell, the lipophilic blocking group is cleaved by non-specific esterases, resulting in a green-fluorescent, charged form, inhibiting it from escaping the live cells. TubulinTracker Green reagent utilizes Oregon Green 488 dye, which absorbs and emits optimally at 494 nm and 522 nm, respectively, and fluorescence can be visualized with standard GFP/FITC filter settings using almost any fluorescent imaging instrument. It can be multiplex with blue, orange, red, deep-red, and near-IR fluorophores.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or Dye: Oregon Green™ 488
Sub-Cellular Localization: Tubulin
Excitation⁄Emission (nm): 494⁄522
Product Line: TubulinTracker™
Product Size: 60 slides
Shipping Condition: Approved for shipment on Wet or Dry Ice

Contents & storage

Contains sufficient material for at least 60 slides at 1 mL per slide and 1X working concentration.

• 1 x 20 µg Tubulin Tracker Green (Oregon Green 488 Taxol, bis-acetate)
• 1 x 1 mL Pluronic F-127, 20% solution in DMSO
• 1 x 77 mg Probenecid

Store at ≤–20°C after recieving.
Desiccate and protect from light.
When stored as directed, product is stable for at least 6 months.

Documents

Manuals & protocols