FM™ 2-10 (<i>N</i>-(3-Triethylammoniumpropyl)-4-(4-(Diethylamino)styryl)Pyridinium Dibromide)

Zitierungen und Referenzen (27)

Invitrogen™

FM™ 2-10 (N-(3-Triethylammoniumpropyl)-4-(4-(Diethylamino)styryl)Pyridinium Dibromide)

Die FM™ 2-10 Membransonde ein ausgezeichnetes Reagenz für die Identifizierung von aktiv feuernden Neuronen und für die Untersuchung der MechanismenWeitere Informationen

Katalognummer	Menge
T7508	5 mg

Katalognummer T7508

Preis (EUR)

449,92

Sonderangebot

Exklusiv online

Endet: 15-Mar-2026

608,00

Ersparnis 158,08 (26%)

Menge:

Preis (EUR)

449,92

Sonderangebot

Exklusiv online

Endet: 15-Mar-2026

608,00

Ersparnis 158,08 (26%)

Die FM™ 2-10 Membransonde ein ausgezeichnetes Reagenz für die Identifizierung von aktiv feuernden Neuronen und für die Untersuchung der Mechanismen von aktivitätsabhängigen Vesikelzyklen. Von diesem wasserlöslichen Farbstoff, der für Zellen ungiftig und in wässrigem Medium praktisch nicht fluoreszierend ist, wird angenommen, dass er in die äußere Schicht der Zellmembran eingefügt wird, wo er intensiv fluoresziert. In einem Neuron, das aktiv Neurotransmitter freigibt, wird der Farbstoff innerhalb der recycelten synaptischen Vesikel verinnerlicht und die Nervenenden werden hell gefärbt.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

FarbeOrange

NachweisverfahrenFluoreszent

Zur Verwendung mit (Geräte)Fluoreszenzmikroskop

ProduktlinieFM

Menge5 mg

VersandbedingungRaumtemperatur

MarkertypFluorescent Dye

ProdukttypSonde

SubCellular LocalizationPlasmamembran

Unit Size5 mg

Inhalt und Lagerung

Lagerung bei Raumtemperatur und vor Licht geschützt

Häufig gestellte Fragen (FAQ)

I want to study endosome trafficking using FM 4-64 dye. Will the label be retained after fixation? And can I label cells that have already been fixed?

No. For that, you would need the FM 4-64FX version. The non-FX version will be lost, leading to loss of much of the specific label and a vast increase in background labeling. The FX version will be fixed in place with formaldehyde. Cells that have been fixed already will not label correctly, so you will need to label the cells live and then fix.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Zitierungen und Referenzen (27)

Zitierungen und Referenzen

Abstract

Ba2+ does not support synaptic vesicle retrieval in rat cerebrocortical synaptosomes.

Authors:Cousin MA, Robinson PJ

Journal:Neurosci Lett

PubMed ID:9754790

To investigate whether any specific requirement for extracellular Ca2+ exists in the control synaptic vesicle retrieval, we examined the ability of the divalent cation Ba2+ to substitute for Ca2+ in both vesicle exocytosis and endocytosis. Ba2+ stimulated glutamate release from rat cerebrocortical synaptosomes. Ba2+-evoked release was inhibited by bafilomycin A1, ... More

Frequency dependence of synaptic vesicle exocytosis in aortic baroreceptor neurons and the role of group III mGluRs.

Authors:Pamidimukkala J, Hay M

Journal:Brain Res

PubMed ID:15051525

Synaptic transmission between baroreceptor afferents and the nucleus tractus solitarius (NTS) is essential for reflex regulation of blood pressure. High frequency stimulation of the afferents in vivo leads to a decrease in synaptic strength and is generally attributed to reduction in presynaptic neurotransmitter release. It has been hypothesized that during ... More

Long-term staining of live Merkel cells with FM dyes.

Authors:Fukuda J, Ishimine H, Masaki Y

Journal:Cell Tissue Res

PubMed ID:12658440

'Live Merkel cells in the skin and hair follicles are known to incorporate a fluorescence dye, quinacrine, which has been utilized to identify and dissect the cells for experiments. Quinacrine fluorescence of the cells is, however, quickly lost and quinacrine-stained Merkel cells soon become difficult to identify in tissue culture. ... More

Two mechanisms of synaptic vesicle recycling in rat brain nerve terminals.

Authors:Cousin MA, Robinson PJ

Journal:J Neurochem

PubMed ID:10987846

'KCl and 4-aminopyridine (4-AP) evoke glutamate release from rat brain cortical nerve terminals by voltage clamping or by Na(+) channel-generated repetitive action potentials, respectively. Stimulation by 4-AP but not KCl is largely mediated by protein kinase C (PKC). To determine whether KCl and 4-AP utilise the same mechanism to release ... More

Calcium triggers calcineurin-dependent synaptic vesicle recycling in mammalian nerve terminals.

Authors:Marks B, McMahon HT

Journal:Curr Biol

PubMed ID:9651678

'BACKGROUND: Following exocytosis at the synapse, synaptic vesicle components are recovered by endocytosis. Morphological analysis has suggested that this occurs by a clathrin-mediated pathway, and the GTPase dynamin is thought to be involved in ''pinching off'' endocytosing vesicles. The finding that the calcium-dependent phosphatase calcineurin can dephosphorylate dynamin and two ... More

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