To make 1X TBS solution dilute concentrate 25X with distilled water. Use 40mL TBS concentrate per 960mL DI water. Measure and pour appropriate volume of 25X TBS concentrate into a mixing flask and add DI water to final volume. Stir briefly. The 1X solution should be pH 7.6±0.2. The pH of the soution may require slight adjustment. Adjust pH with 1N HCl (if pH is high) or 1N NaOH (if pH is low). Note: Tris buffers require the use of a calomel pH or a double junction pH probe to obtain the correct pH reading.