Invitrogen™

CytoVista™ Tissue Clearing Kit

Catalog number: V11322
Invitrogen™

CytoVista™ Tissue Clearing Kit

Catalog number: V11322
Catalog Number
V11322
Unit Size
30 mL
Price (USD)
Price: 469.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
10 mL
Price (USD)
Price: 177.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
V1132230 mL
Price: 469.00
Your Price:
-
V1130410 mL
Price: 177.00
Your Price:
-
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations

The CytoVista Tissue Clearing Kit clears fluorescently labeled tissue to enable sharp and bright 3D fluorescent imaging at any tissue depth. The kit contains both CytoVista Tissue Clearing Reagent and CytoVista Tissue Clearing Enhancer, which together can be used for to clear most fluorescently labeled tissue types. CytoVista Tissue Clearing Reagent or Enhancer can also be used as an imaging solution with stable storage of tissues for weeks with minimal change in tissue morphology or brightness, or specificity of fluorophores.

Features of the CytoVista family of tissue and 3D cell culture clearing reagents include:
• Rapid clearing of fluorescently labeled tissue for 3D imaging
• Minimal shrinkage, expansion, or other morphological changes to tissue
• Simple workflow does not require any special instruments or equipment and is easily adapted to IHC
• Compatible with most fluorophores, including dyes and fluorescent proteins

The CytoVista tissue and 3D cell culture clearing workflow clears tissue or 3D cultured cells with minimal changes to morphology or the sensitivity of detection with almost any fluorophore. With an easy-to-use protocol, whole mouse brain (8-mm section) can be cleared in 24 hours, or a 1-mm section in 2 hours, without the use of any special instruments or equipment.

The CytoVista clearing workflow is compatible with most fluorophores, including fluorescent proteins that are detected with common fluorescent imaging instruments, such as wide-field microscopes, high content analyzers, confocal microscopes, and lightsheet microscopes. For first time users, we recommend the CytoVista Tissue Clearing/Staining Kit (Cat No V11324) or CytoVista 3D Cell Culture Clearing/Staining Kit (Cat No. V11325). These kits contain all necessary reagents for both clearing and IHC staining.

CytoVista clearing technology sufficiently clears tissue for 3D fluorescent imaging, but unlike other clearing techniques does not significantly changes the tissue morphology. Minimal morphological changes, such as shrinkage and contraction, have been observed. For precious samples, the clearing can be reversed, and the tissue can be processed for histology studies, like H&E staining.

For tissues that are easier to clear, like brain, lung, intestine, skin, and tissues of less than 250-µm thickness, treatment with CytoVista Tissue Clearing Reagent is sufficient for 3D imaging. However, tissues of more than 250-µm thickness or that are hard to clear like kidney, liver, heart, and placenta will require an initial treatment with CytoVista Tissue Clearing Reagent followed by treatment with CytoVista Tissue Clearing Enhancer.

CytoVista 3D Cell Culture Clearing Reagent is recommended for spheroids or organoids up to 1 mm in thickness.

For hard tissues like bone, cartilage, and connective tissue, CytoVista Tissue Clearing Reagent is not recommended.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Type
Tissue Clearing Kit
Product Line
CytoVista™
Reagent Type
Cell & Tissue Preparation Reagent
Quantity
30 mL
Shipping Condition
Approved for shipment at Room Temperature or on Wet Ice

Contents & Storage

  • CytoVista Tissue Clearing Reagent, 30 mL
  • CytoVista Tissue Clearing Enhancer, 30 mL
  • Store at room temperature.