Invitrogen™

Dead Cell Apoptosis Kit with Annexin V FITC and PI, for flow cytometry

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Description

This product detects the externalization of phosphatidylserine in apoptotic cells using recombinant annexin V conjugated to green-fluorescent FITC dye and dead cells using propidium iodide (PI). Propidium iodide stains necrotic cells with red fluorescence. After treatment with both probes, apoptotic cells show green fluorescence, dead cells show red and green fluorescence, and live cells show little or no fluorescence.

View a selection guide for all apoptosis assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Fluorescent
Excitation⁄Emission (nm): FITC: 494⁄518,PI: 535⁄617
Flow Cytometer Laser Lines: 488
For Use With (Equipment): Flow Cytometer
Format: Tube(s)
Label or Dye: FITC (Fluorescein),propidium iodide
Number of Reactions: 50
Product Line: Vybrant®
Product Size: 1 kit
Shipping Condition: Wet Ice

Contents & storage

Contains 1 vial of annexin V, FITC conjugate (250 µL ), 1 vial of propidium iodide (PI, 100 µL), and 1 bottle of annexin binding buffer (5X solution, 15 mL).

Store in refrigerator (2–8°C) and protect from light. Store in refrigerator (2–8°C) and protect from light.