Dead Cell Apoptosis Kit with Annexin V FITC and PI, for flow cytometry

Catalog number: V13242

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function | Cellular Imaging | Chromatin Biology | Flow Cytometry | Target-Based ADME⁄Tox Assays

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Description

This product detects the externalization of phosphatidylserine in apoptotic cells using recombinant annexin V conjugated to green-fluorescent FITC dye and dead cells using propidium iodide (PI). Propidium iodide stains necrotic cells with red fluorescence. After treatment with both probes, apoptotic cells show green fluorescence, dead cells show red and green fluorescence, and live cells show little or no fluorescence.

View a selection guide for all apoptosis assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Tube(s)
Label or Dye: Propidium iodide, FITC (Fluorescein)
Product Size: 1 kit
Detection Method: Fluorescent
Number of Reactions: 50
For Use With (Equipment): Flow Cytometer
Excitation⁄Emission (nm): PI: 535⁄617, FITC: 494⁄518
Shipping Condition: Wet Ice

Contents & storage

Contains 1 vial of annexin V, FITC conjugate (250 µL ), 1 vial of propidium iodide (PI, 100 µL), and 1 bottle of annexin binding buffer (5X solution, 15 mL).

Store in refrigerator (2–8°C) and protect from light. Store in refrigerator (2–8°C) and protect from light.

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