Invitrogen™

Vybrant™ Apoptosis Assay Kits for apoptotic and necrotic cell staining

Catalog number: V13243
Invitrogen™

Vybrant™ Apoptosis Assay Kits for apoptotic and necrotic cell staining

Catalog number: V13243
Apoptotic cells undergo several physiological processes, including changes in cell membrane permeability and chromatin condensation, which can be distinguished using different dyes in apoptotic and necrotic cell staining protocols. Vybrant Apoptosis Assay kits offer streptavidin biotin-annexin V, propidium iodide (PI), YO-PRO-1, and Hoechst 33342 dyes to differentiate live from apoptotic or necrotic cells, which can then be visualized using flow cytometry.
 
Catalog Number
V13243
Unit Size
200 assays
Label or Dye
YO-PRO-1, propidium iodide
Description
Vybrant Apoptosis Assay Kit #4
Price (USD)
Price: 279.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptionVybrant Apoptosis Assay Kit #4
FormatTube
Kit ContentsContains 1 vial of YO-PRO-1, and 1 vial of propidium iodide.
Product TypeApoptosis Assay Kit
Flow Cytometer Laser Lines488
Excitation/EmissionYO-PRO-1: 491/509
propidium iodide: 535/617
ColorGreen, Red
Shipping ConditionRoom Temperature
Product LineVybrant
Detection MethodFluorescent
For Use With (Equipment)Flow Cytometer
Label or DyeYO-PRO-1, propidium iodide
Label TypeOther Label(s) or Dye(s)
Storage RequirementsStore in refrigerator (2–8°C) and protect from light
Unit Size200 assays
Catalog Number
Unit Size
200 assays
Label or Dye
Hoechst 33342, propidium iodide
Description
Vybrant Apoptosis Assay Kit #5
Price (USD)
Price: 227.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptionVybrant Apoptosis Assay Kit #5
FormatTube
Kit Contents1 vial of Hoechst 33342 and 1 vial of propidium iodide
Product TypeApoptosis Kit
Flow Cytometer Laser Lines488, UV
Excitation/EmissionHoechst: 350/461
propidium iodide: 535/617
ColorBlue, Red
Shipping ConditionRoom Temperature
Product LineVybrant
Detection MethodFluorescent
For Use With (Equipment)Flow Cytometer
Label or DyeHoechst 33342, propidium iodide
Label TypeOther Label(s) or Dye(s)
Storage RequirementsStore in refrigerator (2–8°C) and protect from light
Unit Size200 assays
Catalog Number
Unit Size
50 assays
Label or Dye
Alexa Fluor™ 350 Streptavidin, propidium iodide, Biotin-X Annexin V
Description
Vybrant Apoptosis Assay Kit #6
Price (USD)
Price: 387.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptionVybrant Apoptosis Assay Kit #6
FormatTube
Kit Contents1 vial of biotin-x annexin V, 1 vial of Alexa Fluor™ 350 streptavidin, 1 vial of propidium iodide, and 1 bottle of 5x annexin binding buffer
Product TypeApoptosis Kit
Flow Cytometer Laser LinesUV, 488
Excitation/EmissionAlexa Fluor™ 346/442: 346
PI: 535/617
ColorBlue, Red
Shipping ConditionWet Ice
Product LineAlexa Fluor™
Detection MethodFluorescent
For Use With (Equipment)Flow Cytometer
Label or DyeAlexa Fluor™ 350 Streptavidin, propidium iodide, Biotin-X Annexin V
Label TypeAlexa Fluor™ Dyes, Biotin & Other Haptens, Other Label(s) or Dye(s)
Storage RequirementsStore in refrigerator (2–8°C) and protect from light
Unit Size50 assays
Showing 3 of 3
Catalog NumberSpecificationsUnit SizeLabel or DyeDescriptionPrice (USD)AvailabilityQuantity
V13243Full specifications
200 assaysYO-PRO-1, propidium iodideVybrant Apoptosis Assay Kit #4
Price: 279.00
Your Price:
***
DescriptionVybrant Apoptosis Assay Kit #4
FormatTube
Kit ContentsContains 1 vial of YO-PRO-1, and 1 vial of propidium iodide.
Product TypeApoptosis Assay Kit
Flow Cytometer Laser Lines488
Excitation/EmissionYO-PRO-1: 491/509
propidium iodide: 535/617
ColorGreen, Red
Shipping ConditionRoom Temperature
Product LineVybrant
Detection MethodFluorescent
For Use With (Equipment)Flow Cytometer
Label or DyeYO-PRO-1, propidium iodide
Label TypeOther Label(s) or Dye(s)
Storage RequirementsStore in refrigerator (2–8°C) and protect from light
Unit Size200 assays
V13244Full specifications
200 assaysHoechst 33342, propidium iodideVybrant Apoptosis Assay Kit #5
Price: 227.00
Your Price:
***
DescriptionVybrant Apoptosis Assay Kit #5
FormatTube
Kit Contents1 vial of Hoechst 33342 and 1 vial of propidium iodide
Product TypeApoptosis Kit
Flow Cytometer Laser Lines488, UV
Excitation/EmissionHoechst: 350/461
propidium iodide: 535/617
ColorBlue, Red
Shipping ConditionRoom Temperature
Product LineVybrant
Detection MethodFluorescent
For Use With (Equipment)Flow Cytometer
Label or DyeHoechst 33342, propidium iodide
Label TypeOther Label(s) or Dye(s)
Storage RequirementsStore in refrigerator (2–8°C) and protect from light
Unit Size200 assays
V23200Full specifications
50 assaysAlexa Fluor™ 350 Streptavidin, propidium iodide, Biotin-X Annexin VVybrant Apoptosis Assay Kit #6
Price: 387.00
Your Price:
***
DescriptionVybrant Apoptosis Assay Kit #6
FormatTube
Kit Contents1 vial of biotin-x annexin V, 1 vial of Alexa Fluor™ 350 streptavidin, 1 vial of propidium iodide, and 1 bottle of 5x annexin binding buffer
Product TypeApoptosis Kit
Flow Cytometer Laser LinesUV, 488
Excitation/EmissionAlexa Fluor™ 346/442: 346
PI: 535/617
ColorBlue, Red
Shipping ConditionWet Ice
Product LineAlexa Fluor™
Detection MethodFluorescent
For Use With (Equipment)Flow Cytometer
Label or DyeAlexa Fluor™ 350 Streptavidin, propidium iodide, Biotin-X Annexin V
Label TypeAlexa Fluor™ Dyes, Biotin & Other Haptens, Other Label(s) or Dye(s)
Storage RequirementsStore in refrigerator (2–8°C) and protect from light
Unit Size50 assays
Showing 3 of 3
Vybrant Apoptosis Assay kits offer quick and convenient cell staining dye-based methodologies for differentiating apoptotic and necrotic versus healthy cells, which can then be visualized using flow cytometry.

The Vybrant Apoptosis Assay Kit #4 detects apoptosis via changes that occur in cell membrane permeability. This kit contains ready-to-use solutions of both YO-PRO-1 and propidium iodide (PI) nucleic acid stains. YO-PRO-1 stain selectively passes through the plasma membranes of apoptotic cells and labels them with moderate green fluorescence. Necrotic cells are stained fluorescent red with PI.

The Vybrant Apoptosis Assay Kit #5, or the Chromatin Condensation/Dead Cell Apoptosis Kit with Hoechst 33342 and PI for Flow Cytometry, provides a rapid and convenient assay for apoptosis based on fluorescence detection of the compacted state of the chromatin in apoptotic cells. This kit contains ready-to-use solutions of blue-fluorescent Hoechst 33342 dye, which stains the condensed chromatin of apoptotic cells more brightly than the chromatin of non-apoptotic cells, and red-fluorescent PI dye, which stains dead cells.

The Vybrant Apoptosis Assay Kit #6 provides a rapid and convenient assay for apoptosis, using the bright, blue-fluorescent Alexa Fluor 350 conjugate of streptavidin in conjunction with biotin-X annexin V to detect apoptotic cell populations either by flow cytometry or imaging. In addition, the kit includes a ready-to-use solution of the fluorescent red PI nucleic acid–binding dye. PI is impermeant to live cells and apoptotic cells, but stains dead cells with red fluorescence, binding tightly to their nucleic acid. The kit contains an ample amount of each reagent for about 50 flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures.

Figures