pAO815 Pichia Expression Vector

Catalog number: V18020

Invitrogen™  Related applications: Protein Expression

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

pAO815 is a Pichia expression vector designed to clone multiple copies of a gene into a single vector. The vector contains a Bgl II site upstream of the 5´ AOX1 gene and a unique BamH I site downstream of the 3´ AOX1 transcription termination (TT) signal. Four steps are required to generate multiple copies of the gene of interest:
1. The gene is cloned into the unique EcoR I site in the vector.
2. The construct is digested with BamH I and Bgl II to release the "expression cassette" containing the AOX1 promoter, gene of interest, and 3´ AOX1 TT.
3. Concatemers of the expression cassette are generated by ligation in vitro.
4. The multiple copies are inserted back into the pAO815 vector and transformed into Pichia. This vector is included in the Multi-Copy Pichia Expression Kit.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Antibiotic Resistance (Bacterial): Ampicillin (AmpR)
Cloning Method: Restriction Enzyme ⁄ MCS
Product Size: 20 µg
Promoter: AOX1
Protein Tag or Fusion: Untagged
Vector Type: pAO

Contents & storage

Store at -20°C.

Documents

Manuals & protocols