Informational:Informational:This.Is.Simple. Reduce assay times with the CellEvent® Kit for flow cytometry
Invitrogen™

Vybrant™ FLICA Caspase Apoptosis Assay Kits for flow cytometry

Catalog number: V35119
Invitrogen™

Vybrant™ FLICA Caspase Apoptosis Assay Kits for flow cytometry

Catalog number: V35119
Quickly detect caspase-mediated apoptosis by flow cytometry with Vybrant FAM caspase assay kits for poly-caspases and caspases 3 and 7. The Vybrant FAM caspase assay kits use fluorescent inhibitor of caspases (FLICA) methodology to detect and report caspase activity. Specifically, these caspase assay kits contain engineered FLICA substrates FAM-VAD-FMK (for poly-caspases), FAM-DEVD-FMK (for caspase-3/7), and FAM-LETD-FMK (for caspase-8). They also include Hoechst 33342 and propidium iodide stains, enabling simultaneous evaluation of membrane permeability and cell cycle by flow cytometry.
 
Catalog Number
V35119
Unit Size
25 assays
Label or Dye
FAM, propidium iodide, Hoechst 33342
Description
Vybrant FAM Caspase-8 Assay Kit
Price (USD)
274.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptionVybrant FAM Caspase-8 Assay Kit
FormatTube
Kit Contents1 vial of FAM-LETD-FMK caspase-8 reagent (FLICA reagent), 1 vial Hoechst 33342, 1 vial of propidium iodide, 1 vial of DMSO, 1 bottle of apoptosis fixative solution, and 1 bottle of apoptosis wash buffer (10X solution).
Product TypeCaspase Assay Kit
Flow Cytometer Laser LinesUV, 488
Excitation/EmissionFAM-LETD-FMDK: 488/530
PI: 535/617
Hoechst 33342: 350/461
Shipping ConditionRoom Temperature
Product LineVybrant
Detection MethodFluorescent
For Use With (Equipment)Flow Cytometer
Label or DyeFAM, propidium iodide, Hoechst 33342
Label TypeOther Label(s) or Dye(s)
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Unit Size25 assays
Catalog Number
Unit Size
25 assays
Label or Dye
FMK, Hoechst 33342, propidium iodide
Description
Vybrant FAM Poly Caspases Assay Kit
Price (USD)
279.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptionVybrant FAM Poly Caspases Assay Kit
FormatTube
Kit Contents1 vial of FAM-VAD-FMK poly caspases reagent (FLICA reagent), 1 vial Hoechst 33342, 1 vial of propidium iodide, 1 vial of DMSO, 1 bottle of apoptosis fixative solution, and 1 bottle of apoptosis wash buffer (10X solution)
Product TypeCaspase Assay Kit
Flow Cytometer Laser LinesUV, 488
Excitation/EmissionFAM-VAD-FMDK: 488/530
PI: 535/617
Hoechst 33342: 350/461
Shipping ConditionRoom Temperature
Product LineVybrant
Detection MethodFluorescent
For Use With (Equipment)Flow Cytometer
Label or DyeFMK, Hoechst 33342, propidium iodide
Label TypeOther Label(s) or Dye(s)
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Unit Size25 assays
Catalog Number
Unit Size
25 assays
Label or Dye
FAM, Hoechst 33342, propidium iodide
Description
Vybrant FAM Caspase-3 and -7 Assay Kit
Price (USD)
278.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptionVybrant FAM Caspase-3 and -7 Assay Kit
FormatTube
Kit Contents1 vial FAM-DEVD-FMK caspase-3 and -7 reagent (FLICA reagent, lyophilized solid), 1 vial of Hoechst 33342 (400 µL, 1 mM in water), 1 vial of PI (1 mL, 250 µg/mL in water), 1 vial of DMSO (0.5 mL), 1 bottle of apoptosis fixative solution (10% formaldehyde with methanol in PBS, 6 mL), and 1 bottle of 10x apoptosis wash buffer.
Product TypeCaspase Assay Kit
Flow Cytometer Laser LinesUV, 488
Excitation/EmissionFAM-DEVD-FMD: 388/530
PI: 535/617
Hoechst 33342: 350/461
Shipping ConditionRoom Temperature
Product LineVybrant
Detection MethodFluorescent
For Use With (Equipment)Flow Cytometer
Label or DyeFAM, Hoechst 33342, propidium iodide
Label TypeOther Label(s) or Dye(s)
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Unit Size25 assays
Showing 3 of 3
Catalog NumberSpecificationsUnit SizeLabel or DyeDescriptionPrice (USD)AvailabilityQuantity
V35119Full specifications
25 assaysFAM, propidium iodide, Hoechst 33342Vybrant FAM Caspase-8 Assay Kit
274.00
Your Price:
***
DescriptionVybrant FAM Caspase-8 Assay Kit
FormatTube
Kit Contents1 vial of FAM-LETD-FMK caspase-8 reagent (FLICA reagent), 1 vial Hoechst 33342, 1 vial of propidium iodide, 1 vial of DMSO, 1 bottle of apoptosis fixative solution, and 1 bottle of apoptosis wash buffer (10X solution).
Product TypeCaspase Assay Kit
Flow Cytometer Laser LinesUV, 488
Excitation/EmissionFAM-LETD-FMDK: 488/530
PI: 535/617
Hoechst 33342: 350/461
Shipping ConditionRoom Temperature
Product LineVybrant
Detection MethodFluorescent
For Use With (Equipment)Flow Cytometer
Label or DyeFAM, propidium iodide, Hoechst 33342
Label TypeOther Label(s) or Dye(s)
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Unit Size25 assays
V35117Full specifications
25 assaysFMK, Hoechst 33342, propidium iodideVybrant FAM Poly Caspases Assay Kit
279.00
Your Price:
***
DescriptionVybrant FAM Poly Caspases Assay Kit
FormatTube
Kit Contents1 vial of FAM-VAD-FMK poly caspases reagent (FLICA reagent), 1 vial Hoechst 33342, 1 vial of propidium iodide, 1 vial of DMSO, 1 bottle of apoptosis fixative solution, and 1 bottle of apoptosis wash buffer (10X solution)
Product TypeCaspase Assay Kit
Flow Cytometer Laser LinesUV, 488
Excitation/EmissionFAM-VAD-FMDK: 488/530
PI: 535/617
Hoechst 33342: 350/461
Shipping ConditionRoom Temperature
Product LineVybrant
Detection MethodFluorescent
For Use With (Equipment)Flow Cytometer
Label or DyeFMK, Hoechst 33342, propidium iodide
Label TypeOther Label(s) or Dye(s)
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Unit Size25 assays
V35118Full specifications
25 assaysFAM, Hoechst 33342, propidium iodideVybrant FAM Caspase-3 and -7 Assay Kit
278.00
Your Price:
***
DescriptionVybrant FAM Caspase-3 and -7 Assay Kit
FormatTube
Kit Contents1 vial FAM-DEVD-FMK caspase-3 and -7 reagent (FLICA reagent, lyophilized solid), 1 vial of Hoechst 33342 (400 µL, 1 mM in water), 1 vial of PI (1 mL, 250 µg/mL in water), 1 vial of DMSO (0.5 mL), 1 bottle of apoptosis fixative solution (10% formaldehyde with methanol in PBS, 6 mL), and 1 bottle of 10x apoptosis wash buffer.
Product TypeCaspase Assay Kit
Flow Cytometer Laser LinesUV, 488
Excitation/EmissionFAM-DEVD-FMD: 388/530
PI: 535/617
Hoechst 33342: 350/461
Shipping ConditionRoom Temperature
Product LineVybrant
Detection MethodFluorescent
For Use With (Equipment)Flow Cytometer
Label or DyeFAM, Hoechst 33342, propidium iodide
Label TypeOther Label(s) or Dye(s)
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Unit Size25 assays
Showing 3 of 3
The Vybrant FAM caspase assay kits for flow cytometry employ a novel approach to detecting active caspases: the kits take advantage of FLICA methodology, which is an affinity label. This affinity label associates a fluoromethyl ketone (FMK) moiety, which can react covalently with a cysteine, with a caspase-specific amino acid sequence. Different amino acid recognition sequences are used to detect different caspases: valine-alanine-aspartic acid (VAD) for poly caspases (including caspase-1, -3, -4, -5, -6, -7, -8, and -9), aspartic acid-glutamic acid-valine-aspartic acid (DEVD) for caspase-3/7, and leucine-glutamic acid-threonine-aspartic acid (LETD) for caspase-8. A carboxyfluorescein (FAM) group is attached as a reporter.

The FLICA reagent is thought to interact with the enzymatic reactive center of an activated caspase via the recognition sequence, and then to attach covalently through the FMK moiety. The FLICA inhibitor is cell permeant and noncytotoxic. Unbound FLICA molecules diffuse out of the cell and are washed away; the remaining fluorescent signal is a direct measure of the amount of active caspase present at the time the inhibitor was added. The approximate excitation and emission peaks of the FLICA, propidium iodide, and Hoechst 33342 reagents are 488 nm/⁄530 nm, 535⁄ nm/6617 nm, and 350 nm/⁄461 nm, respectively. The detection reagents included within the individual Vybrant FAM Poly Caspases Assay, Caspase-3 and -7 Assay, and Caspase-8 Assay apoptosis kits are 488⁄⁄530 FAM-VAD-FMK reagent, 488/⁄530 FAM-DEVD-FMK, and 488/⁄530 FAM-LETD-FMK, respectively.
For Research Use Only. Not for use in diagnostic procedures.

Figures