Vivid Colors™ pcDNA™6.2/EmGFP-Bsd/V5-DEST Vector

Catalog number: V36620

Invitrogen™  Related applications: Cellular Imaging | Gateway Cloning | Protein Assays and Analysis | Protein Expression

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Description

The Vivid Colors™ pcDNA™ 6.2/EmGFP-Bsd/V5-DEST vector allows you to rapidly clone your gene using Gateway® technology and simultaneously express it with Emerald Green Fluorescent Protein (EmGFP) for easy identification of transfected cells. The EmGFP is derived from well-characterized fluorescent proteins of the jellyfish Aequorea victoria (1, 2) and has been humanized for optimal mammalian expression (3) (Figure 1). If cloned with the TAG (amber) codon, your gene will be expressed in its native configuration and fully compatible with Tag-On-Demand" technology. If cloned without a stop codon, a V5-epitope tag will be fused to the carboxy-terminal end of your protein of interest. In either case, expression of EmGFP provides a bright fluorescent marker to easily identify cells co-expressing your protein. This marker allows you to focus your functional analysis on specific cells, or to sort and enrich for transfected cells in a derived population.

The Vivid Colors™ pcDNA™ 6.2/EmGFP-Bsd/V5-DEST vector provides:

• PGK promoter for high-level expression of EmGFP fused to the Blasticidin (bsd)-resistance marker
• CMV promoter for high-level expression of your protein
• V5-epitope tag option to fuse to the C-terminal end of your protein, if desired
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: CMV
Key Function: Reporter Assays
Protein Tag or Fusion: V5 Epitope Tag
Reporter: GFP (EmGFP)
Delivery Method: Transfection
Cloning Method: Gateway®
Selection Agent (Eukaryotic): Blasticidin
Vector Type: pDEST, pcDNA
Constitutive or Inducible System: Constitutive
Product Size: 6 µg

Contents & storage

Each vector is supplied as supercoiled and lyophilized plasmid DNA. Store at -20°C after resuspended. Guaranteed stable for 6 months when properly stored.

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