pMT/BiP/V5-His A, B, & C Drosophila Expression Vectors

Catalog number: V413020

Invitrogen™  Related applications: Protein Expression

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Description

The DES®-Inducible/Secreted Kit provides the vector pMT/BiP/V5-His for inducible, secreted expression of recombinant proteins and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. This vector offers the inducible metallothionein promoter that is induced upon addition of copper sulfate or cadmium chloride. The N-terminal signal sequence from the insect BiP gene is provided to direct the recombinant fusion protein through the secretory pathway of S2 cells into the culture medium. The pMT/BiP/V5-His vector offers the following additional features:

• Small size (3.6 kb) to improve DNA yields and increase subcloning efficiency
• C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody
• C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin

To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the BiP signal sequence.
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Promoter: MT
Vector Type: pMT
Product Size: 20 µg
Cloning Method: Restriction Enzyme ⁄ MCS
Protein Tag or Fusion: His Tag (6x), V5 Epitope Tag

Contents & storage

• 20 µg each of pMT/BiP/V5-His A, B, and C vectors, lyophilized in TE Buffer (pH 8.0)
• 20 µg of pMT/BiP/V5-His/GFP, lyophilized in TE Buffer (pH 8.0)

Store vectors at -20°C.

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Manuals & protocols

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