Catalog number: V50320

Invitrogen™  Related applications: Protein Expression

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pTEF1/Zeo is a 3.6 kb vector designed to allow you to create your own cloning and/or expression vectors that confer resistance to Zeocin™ in both E. coli and yeast strains. The vector contains the Sh ble Zeocin™ resistance gene driven by the Saccharomyces cerevisiae TEF1 promoter for selection in any yeast. In addition, the synthetic EM-7 bacterial promoter is present for selection in E. coli. Several unique restriction sites flank the ZeoCassette™ region to allow easy subcloning of the region or cloning of additional elements into pTEF1/Zeo. This vector is also useful for generating gene disruptions in yeast.
For Research Use Only. Not for use in diagnostic procedures.


Promoter: TEF1
Vector Type: pTEF
Product Size: 20 µg
Cloning Method: Restriction Enzyme ⁄ MCS
Protein Tag or Fusion: Untagged
Selection Agent (Eukaryotic): Zeocin™
Antibiotic Resistance (Bacterial): Zeocin™ (ZeoR)

Contents & storage

20 µg of pTEF1/Zeo is provided supercoiled and lyophilized with 125 mg of Zeocin™ . Store the vector and Zeocin™ at -20°C. All components are guaranteed stable for 6 months when properly stored. See polylinker sequences on the web.


Manuals & protocols

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