pZeoSV2(+) Vector Kit

Catalog number: V85001

Invitrogen™  Related applications: Protein Expression

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Description

pZeoSV2(+) and (-) are 3.5-kb vectors designed for high-level, constitutive expression in mammalian cell lines that express the SV40 large T antigen. The Zeocin™ resistance gene provides fast and efficient selection in E. coliand mammalian cells that do not express the SV40 large T antigen. The vectors have the following features which make them efficient, easy-to-use tools:

• SV40 enhancer-promoter and origin for high-level, constitutive expression and replication in mammalian cells
• BGH polyA signal for efficient processing of mRNA transcripts
• Multiple cloning site in the forward (+) and reverse (-) orientations for easier cloning
• Small size for efficient cloning
• f1 origin for the rescue of single-strand DNA (sense strand)
• pUC origin of replication for growth in E. coli

Expression of the Zeocin™ resistance gene is driven by the CMV promoter in mammalian cell lines and by the synthetic EM-7 promoter in E. coli. pZeoSV2 can be used in transient expression assays as well as to create stable cell lines.
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Promoter: SV40
Key Function: Constitutive Expression
Protein Tag or Fusion: Untagged
Delivery Method: Transfection
Cloning Method: Restriction Enzyme ⁄ MCS
Selection Agent (Eukaryotic): Zeocin™
Vector Type: pZeo
Constitutive or Inducible System: Constitutive
Product Size: 1 kit

Contents & storage

20 µg pZeoSV2 (+) or (-) and 20 µg pZeoSV2/lacZ control vector (20 µg) are supplied supercoiled and lyophilized, 3 x 1.25 ml Zeocin™ is supplied at 100 mg/ml. Store all components at -20°C. Zeocin™ should be stored away from light. Vectors and Zeocin™ are guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols