pTracer™-SV40 Mammalian Expression Vector - FAQs

View additional product information for pTracer™-SV40 Mammalian Expression Vector - FAQs (V87120)

4 product FAQs found

I used one of your pTracer vectors and do not see any fluorescence. What could have happened?

Here are possible causes and solutions:

- High background fluorescence due to riboflavin in the culture medium-replace medium with 1X PBS to eliminate background fluorescence.
- A filter set was used that did not allow excitation at the optimal wavelength or permit detection of the emitted fluorescence-use the XF76 filter from Omega Optical.
- Transfection efficiency is too low to allow detection of transfected cells-optimize your transfection conditions or try another method.
- Expression of Cycle 3 GFP may be low depending on the cell line used-in HEK-293 cells, maximum fluorescence was observed 72 hours post-transfection.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

I sequenced one of your vectors after PCR amplification and observed a difference from what is provided online (or in the manual). Should I be concerned?

Our vectors have not been completely sequenced. Your sequence data may differ when compared to what is provided. Known mutations that do not affect the function of the vector are annotated in public databases.

Are your vectors routinely sequenced?

No, our vectors are not routinely sequenced. Quality control and release criteria utilize other methods.

How was the reference sequence for your vectors created?

Sequences provided for our vectors have been compiled from information in sequence databases, published sequences, and other sources.