pTracer™-CMV2 Kit - FAQs

I used one of your pTracer vectors and do not see any fluorescence. What could have happened?

Here are possible causes and solutions:

- High background fluorescence due to riboflavin in the culture medium-replace medium with 1X PBS to eliminate background fluorescence.
- A filter set was used that did not allow excitation at the optimal wavelength or permit detection of the emitted fluorescence-use the XF76 filter from Omega Optical.
- Transfection efficiency is too low to allow detection of transfected cells-optimize your transfection conditions or try another method.
- Expression of Cycle 3 GFP may be low depending on the cell line used-in HEK-293 cells, maximum fluorescence was observed 72 hours post-transfection.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.