pEF1/His A, B, & C Mammalian Expression Vectors

Catalog number: V92220

Invitrogen™  Related applications: Protein Expression

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Description

All pEF or pUB vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: EF-1α
Key Function: Constitutive Expression
Protein Tag or Fusion: His Tag (6x), Xpress Epitope Tag
Delivery Method: Transfection
Cloning Method: Restriction Enzyme ⁄ MCS
Selection Agent (Eukaryotic): Geneticin® (G-418)
Vector Type: pEF
Cleavage: EK (Enterokinase) Recognition Site
Constitutive or Inducible System: Constitutive
Product Size: 20 µg

Contents & storage

Vectors (and appropriate expression control) are supplied supercoiled and lyophilized. Stored at -20°C. All vectors are guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols