Invitrogen™

pEXP-5-CT/TOPO™ TA Expression Kit

Catalog number:  V96006

Related applications:

Protein Expression

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Description

The pEXP5-CT/TOPO® TA Expression Kit provides a highly efficient, five-minute, one-step cloning strategy for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for T7-based, high-level expression of recombinant fusion proteins in the Expressway™ Maxi Cell-Free E. coli Expression System or for inducible expression in E. coli. No ligase, post-PCR procedures, or PCR primers containing special sequences are required to generate the expression construct.

Features of the pEXP5-CT/TOPO® vector include:

• Bacteriophage T7 promoter for high-level, inducible expression of the recombinant protein of interest
• C-terminal fusion tag for detection and purification of recombinant fusion proteins
• TOPO® Cloning site for rapid and efficient cloning of Taq-amplified PCR products
• Ampicillin resistance gene for selection in E. coli
• pUC origin for high-copy replication and maintenance of the plasmid in E. coli
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Antibiotic Resistance Bacterial: Ampicillin (AmpR)
Bacterial or Yeast Strain: TOP10
Cloning Method: TOPO®-TA
Expression Mechanism: Cell-Based Expression
Expression System: E. coli
Product Line: Expressway™, TOPO®
Promoter: T7
Selection Agent (Eukaryotic): None
Vector: pEXP, TOPO-TA Vectors
Protein Tag: His Tag (6x)

Contents & storage

The pEXP-5-CT/TOPO® TA Expression Kit includes vector, 10X PCR Buffer, dNTP Mix, Salt Solution, Water, T7 Forward Primer, T7 Term Reverse Primer, Control PCR Primers, and Control PCR Template.