WesternDot™ 800 mCherry Rat Monoclonal Antibody (VIVID™ Technology)

Catalog number:  W10831


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This WesternDot® affinity-purified rat monoclonal antibody against mCherry Fluorescent Protein reliably detects mCherry protein in western blot applications without the need for a secondary antibody. WesternDot® antibodies deliver superior signal-to-noise ratios and exhibit comparable sensitivity to ECL-based chemiluminescent methods in western blot applications. No stripping and re-probing or multiple blots are required for multiple protein detection as commonly practiced with ECL or colorimetric detection. Different colors of WesternDot® antibodies can be applied to a single blot and multiple proteins detected simultaneously using standard gel or blot imaging platforms. Our WesternDot® antibodies use Qdot® nanocrystals enhanced with VIVID® technology, which are brighter than the original Qdot® reagents. Additionally, they have reduced intensity differences between the different colors, simplifying multiplex applications.
Specific—no cross reactivity with Emerald GFP, TagGFP, mKate2, or TagRFP
Up to 3-color detection—combine WesternDot® 800 with WesternDot® 585 and WesternDot® 655 antibodies
Reduce time to result—no secondary antibody required, eliminate cumbersome HRP and ECl optimization
Easy to switch—WesternDot® antibodies use standard membranes, blocking solutions, and buffers
WesternDot®800 antibody excitation/emission: 405-485/800 nm
Host species and isotype: The host species of this mCherry antibody is rat , and the isotype is IgG2a

Because of its improved brightness, superior photostability, and extremely rapid maturation rate, the mCherry monomeric red fluorescent protein is becoming the red fluorescent protein of choice for monitoring physiological processes and detecting transgenic expression.

Learn more about WesternDot® antibodies
For Research Use Only. Not for use in diagnostic procedures.


Clonality: Monoclonal
Host Species: Rat
Label or Dye: Qdot® 800
Product Line: VIVID®, WesternDot®
Product Size: 25 blots
Validated Application: Western Blot

Contents & storage

Store in refrigerator (2–8°C). Do not freeze.


Manuals & protocols

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