WesternDot® 625 His-Tag Mouse Monoclonal Antibody (VIVID® Technology)

Catalog number: W10833

Molecular Probes™

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Description

This WesternDot® affinity-purified mouse histidine-tagged monoclonal antibody specifically detects the 6xHis epitope tag in western blot applications without the need for a secondary antibody. The His-tagged expression system is widely used in the production of recombinant protein.

WesternDot® antibodies deliver superior signal-to-noise ratios and exhibit comparable sensitivity to ECL-based chemiluminescent methods in western blot applications. No stripping and re-probing or multiple blots are required for multiple protein detection as commonly practiced with ECL or colorimetric detection. Different colors of WesternDot® antibodies can be applied to a single blot and multiple proteins detected simultaneously using standard gel or blot imaging platforms. Our WesternDot® antibodies use Qdot® nanocrystals enhanced with VIVID® technology, which are brighter than the original Qdot® reagents. Additionally, they have reduced intensity differences between the different colors, simplifying multiplex applications.

Easy to detect on ethidium bromide gel doc imaging systems
Reduce time to result—no secondary antibody needed, eliminate cumbersome HRP and ECl optimization
Easy to switch—WesternDot® antibodies use standard membranes, blocking solutions, and buffers
WesternDot® 625 antibody excitation/emission: 405-485/625 nm
Host species and isotype: The host species of this histidine-tagged antibody is mouse, and the isotype is IgG1κ
Clone ID of monoclonal antibody (mAb): The monoclonal antibody clone is 4A12E4
Reactivity: Confirmed reactivity for TS-His fusion protein

Learn more about WesternDot® antibodies
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Clonality: Monoclonal
Host Species: Mouse
Label or Dye: Qdot® 625
Product Size: 25 blots
Validated Application: Western Blot

Contents & storage

Store in refrigerator (2–8°C). Do not freeze.

Documents

Manuals & protocols

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