WesternDot® 800 His-Tag Mouse Monoclonal Antibody (VIVID® Technology)

Catalog number: W10835

Molecular Probes™

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Description

This WesternDot® affinity-purified mouse histidine-tagged monoclonal antibody specifically detects the 6xHis epitope tag in western blot applications without the need for a secondary antibody. The His-tagged expression system is widely used in the production of recombinant protein.

WesternDot® antibodies deliver superior signal-to-noise ratios and exhibit comparable sensitivity to ECL-based chemiluminescent methods in western blot applications. No stripping and re-probing or multiple blots are required for multiple protein detection as commonly practiced with ECL or colorimetric detection. Different colors of WesternDot® antibodies can be applied to a single blot and multiple proteins detected simultaneously using standard gel or blot imaging platforms. Our WesternDot® antibodies use Qdot® nanocrystals enhanced with VIVID® technology, which are brighter than the original Qdot® reagents. Additionally, they have reduced intensity differences between the different colors, simplifying multiplex applications.

Reduce time to result—no secondary antibody needed and eliminate cumbersome HRP and ECL optimization
Easy to switch—WesternDot® antibodies use standard membranes, blocking solutions, and buffers
Up to 3-color detection—combine WesternDot® 800 with WesternDot® 585 and WesternDot® 655 antibodies
WesternDot® 800 antibody excitation/emission: 405-485/800 nm
Host species and isotype: The host species of this histidine-tagged antibody is mouse, and the isotype is IgG1κ
Clone ID of monoclonal antibody (mAb): The monoclonal antibody clone is 4A12E4
Reactivity: Confirmed reactivity for TS-His fusion protein

Learn more about WesternDot® antibodies
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Clonality: Monoclonal
Host Species: Mouse
Label or Dye: Qdot® 800
Product Size: 25 blots
Validated Application: Western Blot

Contents & storage

Store in refrigerator (2–8°C). Do not freeze.

Documents

Manuals & protocols

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