Obligate multivalent recognition of cell surface tomoregulin following selection from a multivalent phage antibody library.
AuthorsHeitner T, Satozawa N, McLean K, Vogel D, Cobb RR, Liu B, Mahmoudi M, Finster S, Larsen B, Zhu Y, Zhou H, Müller-Tiemann B, Monteclaro F, Zhao XY, Light DR
JournalJ Biomol Screen
PubMed ID17092910
'A therapeutic antibody candidate (AT-19) isolated using multivalent phage display binds native tomoregulin (TR) as a mul-timer not as a monomer. This report raises the importance of screening and selecting phage antibodies on native antigen and reemphasizes the possibility that potentially valuable antibodies are discarded when a monomeric phage display ... More
N-glycans are direct determinants of CFTR folding and stability in secretory and endocytic membrane traffic.
'N-glycosylation, a common cotranslational modification, is thought to be critical for plasma membrane expression of glycoproteins by enhancing protein folding, trafficking, and stability through targeting them to the ER folding cycles via lectin-like chaperones. In this study, we show that N-glycans, specifically core glycans, enhance the productive folding and conformational ... More
The carboxyl terminus of the alpha-subunit of the amiloride-sensitive epithelial sodium channel binds to F-actin.
AuthorsMazzochi C, Bubien JK, Smith PR, Benos DJ
JournalJ Biol Chem
PubMed ID16356937
'The activity of the amiloride-sensitive epithelial sodium channel (ENaC) is modulated by F-actin. However, it is unknown if there is a direct interaction between alpha-ENaC and actin. We have investigated the hypothesis that the actin cytoskeleton directly binds to the carboxyl terminus of alpha-ENaC using a combination of confocal microscopy, ... More
Nanotubular highways for intercellular organelle transport.
AuthorsRustom A, Saffrich R, Markovic I, Walther P, Gerdes HH
JournalScience
PubMed ID14963329
Cell-to-cell communication is a crucial prerequisite for the development and maintenance of multicellular organisms. To date, diverse mechanisms of intercellular exchange of information have been documented, including chemical synapses, gap junctions, and plasmodesmata. Here, we describe highly sensitive nanotubular structures formed de novo between cells that create complex networks. These ... More
Phosphoglycerate mutase affects Stenotrophomonas maltophilia attachment to biotic and abiotic surfaces.
AuthorsRamos-Hegazy L, Chakravarty S, Anderson GG
Journal
PubMed ID31430538
Stenotrophomonas maltophilia biofilm formation is of increasing medical concern, particularly for lung infections. However, the molecular mechanisms facilitating the biofilm lifestyle in S. maltophilia are poorly understood. We generated and screened a transposon mutant library for mutations that lead to altered biofilm formation compared to wild type. One of these mutations, ... More
ORF7 of varicella-zoster virus is a neurotropic factor.
AuthorsSelariu A, Cheng T, Tang Q, Silver B, Yang L, Liu C, Ye X, Markus A, Goldstein RS, Cruz-Cosme RS, Lin Y, Wen L, Qian H, Han J, Dulal K, Huang Y, Li Y, Xia N, Zhu H,
JournalJ Virol
PubMed ID22674980
Varicella-zoster virus (VZV) is the causative agent of chickenpox and herpes zoster (shingles). After the primary infection, the virus remains latent in sensory ganglia and reactivates upon weakening of the cellular immune system due to various conditions, erupting from sensory neurons and infecting the corresponding skin tissue. The current varicella ... More
Tetanus toxin and botulinum toxin a utilize unique mechanisms to enter neurons of the central nervous system.
AuthorsBlum FC, Chen C, Kroken AR, Barbieri JT,
JournalInfect Immun
PubMed ID22392932
Botulinum neurotoxins (BoNTs) and tetanus neurotoxin (TeNT) are the most toxic proteins for humans. While BoNTs cause flaccid paralysis, TeNT causes spastic paralysis. Characterized BoNT serotypes enter neurons upon binding dual receptors, a ganglioside and a neuron-specific protein, either synaptic vesicle protein 2 (SV2) or synaptotagmin, while TeNT enters upon ... More
4D super-resolution microscopy with conventional fluorophores and single wavelength excitation in optically thick cells and tissues.
Optical super-resolution imaging of fluorescently stained biological samples is rapidly becoming an important tool to investigate protein distribution at the molecular scale. It is therefore important to develop practical super-resolution methods that allow capturing the full three-dimensional nature of biological systems and also can visualize multiple protein species in the ... More
Assessment of golgi apparatus versus plasma membrane-localized multi-drug resistance-associated protein 1.
AuthorsKaufmann AM, Toro-Ramos AJ, Krise JP,
JournalMol Pharm
PubMed ID18557629
Traditionally, proteins belonging to the ATP-binding cassette superfamily have been thought to function exclusively at the plasma membrane (PM) of cells. We have previously shown multidrug resistance-associated protein 1 (MRP1) to reside on the Golgi apparatus of the multidrug resistant (MDR) human leukemic cell line HL-60 (HL-60/ADR); however, neither the ... More
Lipids isolated from bone induce the migration of human breast cancer cells.
AuthorsSilva J, Dasgupta S, Wang G, Krishnamurthy K, Ritter E, Bieberich E
JournalJ Lipid Res
PubMed ID16439808
Bone is the most common site to which breast cancer cells metastasize. We found that osteoblast-like MG63 cells and human bone tissue contain the bile acid salt sodium deoxycholate (DC). MG63 cells take up and accumulate DC from the medium, suggesting that the bone-derived DC originates from serum. DC released ... More
Copper and zinc cause delivery of the prion protein from the plasma membrane to a subset of early endosomes and the Golgi.
AuthorsBrown LR, Harris DA
JournalJ Neurochem
PubMed ID14511113
The cellular isoform of prion protein (PrPC) is a plasma membrane glycoprotein whose conformational conversion into PrPSc is the central molecular event in the propagation of infectious prions. However, the physiological function of PrPC has remained uncertain. The finding that PrPC binds copper ions with low micromolar affinity, coupled with ... More
Glycoproteomic probes for fluorescent imaging of fucosylated glycans in vivo.
AuthorsSawa M, Hsu TL, Itoh T, Sugiyama M, Hanson SR, Vogt PK, Wong CH
JournalProc Natl Acad Sci U S A
PubMed ID16895981
Glycomics is emerging as a new field for the biology of complex glycoproteins and glycoconjugates. The lack of versatile glycan-labeling methods has presented a major obstacle to visualizing at the cellular level and studying glycoconjugates. To address this issue, we developed a fluorescent labeling technique based on the Cu(I)-catalyzed [3 ... More
Alkynyl sugar analogs for the labeling and visualization of glycoconjugates in cells.
AuthorsHsu TL, Hanson SR, Kishikawa K, Wang SK, Sawa M, Wong CH
JournalProc Natl Acad Sci U S A
PubMed ID17296930
Developing tools for investigating the cellular activity of glycans will help to delineate the molecular basis for aberrant glycosylation in pathological processes such as cancer. Metabolic oligosaccharide engineering, which inserts sugar-reporting groups into cellular glycoconjugates, represents a powerful method for imaging the localization, trafficking, and dynamics of glycans and isolating ... More
Pancreatic acinar cells express vesicle-associated membrane protein 2- and 8-specific populations of zymogen granules with distinct and overlapping roles in secretion.
AuthorsWeng N, Thomas DD, Groblewski GE
JournalJ Biol Chem
PubMed ID17272274
Previous studies have demonstrated roles for vesicle-associated membrane protein 2 (VAMP 2) and VAMP 8 in Ca(2+)-regulated pancreatic acinar cell secretion, however, their coordinated function in the secretory pathway has not been addressed. Here we provide evidence using immunofluorescence microscopy, cell fractionation, and SNARE protein interaction studies that acinar cells ... More
Plasma membrane topology of syncytial domains of herpes simplex virus type 1 glycoprotein K (gK): the UL20 protein enables cell surface localization of gK but not gK-mediated cell-to-cell fusion.
AuthorsFoster TP, Alvarez X, Kousoulas KG
JournalJ Virol
PubMed ID12477855
Most spontaneously occurring mutations that cause extensive herpes simplex virus type 1 (HSV-1)-induced cell fusion are single amino acid changes within glycoprotein K (gK). Despite the strong genetic association of gK with virus-induced cell fusion, its direct involvement in cellular membrane fusion has been controversial, largely due to previously unsuccessful ... More
Novel localization of Rab3D in rat intestinal goblet cells and Brunner's gland acinar cells suggests a role in early Golgi trafficking.
AuthorsValentijn JA, van Weeren L, Ultee A, Koster AJ
JournalAm J Physiol Gastrointest Liver Physiol
PubMed ID17395899
Rab3D is a small GTP-binding protein that associates with secretory granules of endocrine and exocrine cells. The physiological role of Rab3D remains unclear. While it has initially been implicated in the control of regulated exocytosis, recent deletion-mutation studies have suggested that Rab3D is involved in the biogenesis of secretory granules. ... More
A novel celecoxib analog UTX-121 inhibits HT1080 cell invasion by modulating membrane-type 1 matrix metalloproteinase.
AuthorsYamahana H, Takino T, Endo Y, Yamada H, Suzuki T, Uto Y
JournalBiochem Biophys Res Commun
PubMed ID31629465
'We designed and synthesized a celecoxib derivative UTX-121 to enhance its anti-tumor activity. Similar to celecoxib, this compound could also inhibit matrix metalloproteinase (MMP)-9 activity. In addition, UTX-121 suppressed membrane-type 1 MMP (MT1-MMP)-mediated pro-MMP-2 activation by disturbing the cell surface expression of MT1-MMP. UTX-121 also impeded the glycosylation of cell ... More
AuthorsHe J, Li T, Próchnicki T, Horvath G, Latz E, Takeoka S
JournalBiochem Biophys Rep
PubMed ID31011633
'Lysine (K) type cationic lipid with a propyl spacer and ditetradecyl hydrophobic moieties composing liposomes, K3C14, previously studied for gene delivery, were reported to activate the NLRP3 inflammasomes in human macrophages via the conventional phagolysosomal pathway. In this study, K3C16, a propyl spacer bearing lysine type lipids with dihexadecyl moieties ... More
Yap regulates skeletal muscle fatty acid oxidation and adiposity in metabolic disease.
Authors
JournalNat Commun
PubMed ID34001905
Selective intrarenal delivery of mesenchymal stem cell-derived extracellular vesicles attenuates myocardial injury in experimental metabolic renovascular disease.
Authors
JournalBasic Res Cardiol
PubMed ID31938859
Self-assembling chimeric polypeptide-doxorubicin conjugate nanoparticles that abolish tumours after a single injection.
Authors
JournalNat Mater
PubMed ID19898461
Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens.
Authors
JournalNature
PubMed ID27383987
Epigenetic repression of cardiac progenitor gene expression by Ezh2 is required for postnatal cardiac homeostasis.
Authors
JournalNat Genet
PubMed ID22267199
Combination Chemo-Immunotherapy for Pancreatic Cancer Using the Immunogenic Effects of an Irinotecan Silicasome Nanocarrier Plus Anti-PD-1.
Authors
JournalAdv Sci (Weinh)
PubMed ID33747719
Chronic activation of hexosamine biosynthesis in the heart triggers pathological cardiac remodeling.
Authors
JournalNat Commun
PubMed ID32286306
Type V Collagen in Scar Tissue Regulates the Size of Scar after Heart Injury.
Authors
JournalCell
PubMed ID32621799
Cross-linking peptide and repurposed drugs inhibit both entry pathways of SARS-CoV-2.
Authors
JournalNat Commun
PubMed ID33750821
Natural product fragment combination to performance-diverse pseudo-natural products.
Authors
JournalNat Commun
PubMed ID33767198
A simple and robust fluorescent labeling method to quantify trophoblast fusion.
AuthorsZhang Y, Yang H
JournalPlacenta
PubMed ID30827351
Trophoblast fusion into syncytiotrophoblasts is a specialized yet enigmatic cellular process, which is essential for placental development and function. To facilitate mechanistic understanding of this critical process, here we re-purposed a widely used fluorescent membrane potential dye, Di-8-ANEPPS, to stably label the plasma membrane of live BeWo trophoblast cells. Compared ... More
Dynamic Transcriptional Responses to Injury of Regenerative and Non-regenerative Cardiomyocytes Revealed by Single-Nucleus RNA Sequencing.
AuthorsCui M, Wang Z, Chen K, Shah AM, Tan W, Duan L, Sanchez-Ortiz E, Li H, Xu L, Liu N, Bassel-Duby R, Olson EN
JournalDev Cell
PubMed ID32220304
The adult mammalian heart is incapable of regeneration following injury. In contrast, the neonatal mouse heart can efficiently regenerate during the first week of life. The molecular mechanisms that mediate the regenerative response and its blockade in later life are not understood. Here, by single-nucleus RNA sequencing, we map the ... More
Glycine Enhances Satellite Cell Proliferation, Cell Transplantation, and Oligonucleotide Efficacy in Dystrophic Muscle.
AuthorsLin C, Han G, Ning H, Song J, Ran N, Yi X, Seow Y, Yin H
JournalMol Ther
PubMed ID32209436
The need to distribute therapy evenly systemically throughout the large muscle volume within the body makes Duchenne muscular dystrophy (DMD) therapy a challenge. Cell and exon-skipping therapies are promising but have limited effects, and thus enhancing their therapeutic potency is of paramount importance to increase the accessibility of these therapies ... More
Group A Streptococcus establishes pharynx infection by degrading the deoxyribonucleic acid of neutrophil extracellular traps.
AuthorsTanaka M, Kinoshita-Daitoku R, Kiga K, Sanada T, Zhu B, Okano T, Aikawa C, Iida T, Ogura Y, Hayashi T, Okubo K, Kurosawa M, Hirahashi J, Suzuki T, Nakagawa I, Nangaku M, Mimuro H
JournalSci Rep
PubMed ID32094510
Group A Streptococcus (GAS) secretes deoxyribonucleases and evades neutrophil extracellular killing by degrading neutrophil extracellular traps (NETs). However, limited information is currently available on the interaction between GAS and NETs in the pathogenicity of GAS pharyngitis. In this study, we modified a mouse model of GAS pharyngitis and revealed an ... More
The HopQ-CEACAM Interaction Controls CagA Translocation, Phosphorylation, and Phagocytosis of
AuthorsBehrens IK, Busch B, Ishikawa-Ankerhold H, Palamides P, Shively JE, Stanners C, Chan C, Leung N, Gray-Owen S, Haas R
JournalmBio
PubMed ID32019805
The
Ovine Herpesvirus 2 Encodes a Previously Unrecognized Protein, pOv8.25, That Targets Mitochondria and Triggers Apoptotic Cell Death.
AuthorsShrestha N, Tobler K, Uster S, Sigrist-Nagy R, Hierweger MM, Ackermann M
JournalJ Virol
PubMed ID32024777
Malignant catarrhal fever (MCF) is a rare but frequently lethal disease of certain cloven-hoofed animals. At least 10 different viruses, all members of the
Skeletal muscle lipid droplets are resynthesized before being coated with perilipin proteins following prolonged exercise in elite male triathletes.
AuthorsJevons EFP, Gejl KD, Strauss JA, Ørtenblad N, Shepherd SO
JournalAm J Physiol Endocrinol Metab
PubMed ID31935113
Intramuscular triglycerides (IMTG) are a key substrate during prolonged exercise, but little is known about the rate of IMTG resynthesis in the postexercise period. We investigated the hypothesis that the distribution of the lipid droplet (LD)-associated perilipin (PLIN) proteins is linked to IMTG storage following exercise. Fourteen elite male triathletes ... More
Multinucleated polyploid cardiomyocytes undergo an enhanced adaptability to hypoxia via mitophagy.
AuthorsJiang YH, Wang HL, Peng J, Zhu Y, Zhang HG, Tang FQ, Jian Z, Xiao YB
JournalJ Mol Cell Cardiol
PubMed ID31783035
There is a large subpopulation of multinucleated polyploid cardiomyocytes (M*Pc CMs) in the adult mammalian heart. However, the pathophysiological significance of increased M*Pc CMs in heart disease is poorly understood. We sought to determine the pathophysiological significance of increased M*Pc CMs during hypoxia adaptation. ... More