Informational:Please note that at certain times of the year Bis-Tris protein gel boxes may arrive inside insulated coolers containing ambient temperature gel packs.
Invitrogen™

NuPAGE™ 8%, Bis-Tris, 1.0 mm, Midi Protein Gels

Catalog number: WG1003BOX
Invitrogen™

NuPAGE™ 8%, Bis-Tris, 1.0 mm, Midi Protein Gels

Catalog number: WG1003BOX
Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity. These precast gels are ideal for applications where protein integrity is crucial. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important.
 
Catalog Number
WG1003BOX
Unit Size
10 gels (1 box)
Wells
26-well
Price (USD)
Price: 192.00
Your Price:
Availability
***
Full specifications
Quantity
AdaptersNo Adapters
For Use With (Equipment)XCell4 SureLock Midi, SureLock Tandem Midi Gel Tank
Gel Percentage8%
Gel SizeMidi
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 15 µL
Separation Range3.5 to 260 kd
Separation TypeDenaturing
Shelf Life12 Months
Wells26-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
Catalog Number
Unit Size
10 gels (1 box)
Wells
12+2-well
Price (USD)
Price: 192.00
Your Price:
Availability
***
Full specifications
Quantity
AdaptersNo Adapters
For Use With (Equipment)XCell4 SureLock Midi, SureLock Tandem Midi Gel Tank
Gel Percentage8%
Gel SizeMidi
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading Volume45 µL + 15 µL
Separation Range3.5 to 260 kd
Separation TypeDenaturing
Shelf Life12 Months
Wells12+2-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
Catalog Number
Unit Size
10 gels (1 box)
Wells
12+2-well, w/adapters
Price (USD)
Price: 202.00
Your Price:
Availability
***
Full specifications
Quantity
AdaptersIncludes Adapters
For Use With (Equipment)Bio-Rad Criterion Cell
Gel Percentage8%
Gel SizeMidi
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading Volume45 µL + 15 µL
Separation Range3.5 to 260 kd
Separation TypeDenaturing
Shelf Life12 Months
Wells12+2-well, w/adapters
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
Catalog Number
Unit Size
10 gels (1 box)
Wells
20-well
Price (USD)
Price: 192.00
Your Price:
Availability
***
Full specifications
Quantity
AdaptersNo Adapters
For Use With (Equipment)XCell4 SureLock Midi, SureLock Tandem Midi Gel Tank
Gel Percentage8%
Gel SizeMidi
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 25 µL
Separation Range3.5 to 260 kd
Separation TypeDenaturing
Shelf Life12 Months
Wells20-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
Catalog Number
Unit Size
10 gels (1 box)
Wells
20-well, w/adapters
Price (USD)
Price: 202.00
Your Price:
Availability
***
Full specifications
Quantity
AdaptersIncludes Adapters
For Use With (Equipment)Bio-Rad Criterion Cell
Gel Percentage8%
Gel SizeMidi
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 25 µL
Separation Range3.5 to 260 kd
Separation TypeDenaturing
Shelf Life12 Months
Wells20-well, w/adapters
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
Catalog Number
Unit Size
10 gels (1 box)
Wells
26-well, w/adapters
Price (USD)
Price: 202.00
Your Price:
Availability
***
Full specifications
Quantity
AdaptersIncludes Adapters
For Use With (Equipment)Bio-Rad Criterion Cell
Gel Percentage8%
Gel SizeMidi
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 15 µL
Separation Range3.5 to 180 kd
Separation TypeDenaturing
Shelf Life12 Months
Wells26-well, w/adapters
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
Showing 6 of 6
Catalog NumberSpecificationsUnit SizeWellsPrice (USD)AvailabilityQuantity
WG1003BOXFull specifications
10 gels (1 box)26-well
Price: 192.00
Your Price:
***
AdaptersNo Adapters
For Use With (Equipment)XCell4 SureLock Midi, SureLock Tandem Midi Gel Tank
Gel Percentage8%
Gel SizeMidi
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 15 µL
Separation Range3.5 to 260 kd
Separation TypeDenaturing
Shelf Life12 Months
Wells26-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
WG1001BOXFull specifications
10 gels (1 box)12+2-well
Price: 192.00
Your Price:
***
AdaptersNo Adapters
For Use With (Equipment)XCell4 SureLock Midi, SureLock Tandem Midi Gel Tank
Gel Percentage8%
Gel SizeMidi
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading Volume45 µL + 15 µL
Separation Range3.5 to 260 kd
Separation TypeDenaturing
Shelf Life12 Months
Wells12+2-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
WG1001AFull specifications
10 gels (1 box)12+2-well, w/adapters
Price: 202.00
Your Price:
***
AdaptersIncludes Adapters
For Use With (Equipment)Bio-Rad Criterion Cell
Gel Percentage8%
Gel SizeMidi
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading Volume45 µL + 15 µL
Separation Range3.5 to 260 kd
Separation TypeDenaturing
Shelf Life12 Months
Wells12+2-well, w/adapters
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
WG1002BOXFull specifications
10 gels (1 box)20-well
Price: 192.00
Your Price:
***
AdaptersNo Adapters
For Use With (Equipment)XCell4 SureLock Midi, SureLock Tandem Midi Gel Tank
Gel Percentage8%
Gel SizeMidi
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 25 µL
Separation Range3.5 to 260 kd
Separation TypeDenaturing
Shelf Life12 Months
Wells20-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
WG1002AFull specifications
10 gels (1 box)20-well, w/adapters
Price: 202.00
Your Price:
***
AdaptersIncludes Adapters
For Use With (Equipment)Bio-Rad Criterion Cell
Gel Percentage8%
Gel SizeMidi
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 25 µL
Separation Range3.5 to 260 kd
Separation TypeDenaturing
Shelf Life12 Months
Wells20-well, w/adapters
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
WG1003AFull specifications
10 gels (1 box)26-well, w/adapters
Price: 202.00
Your Price:
***
AdaptersIncludes Adapters
For Use With (Equipment)Bio-Rad Criterion Cell
Gel Percentage8%
Gel SizeMidi
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 15 µL
Separation Range3.5 to 180 kd
Separation TypeDenaturing
Shelf Life12 Months
Wells26-well, w/adapters
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
Showing 6 of 6

Features of NuPAGE Bis-Tris gels:
Better protein integrity—optimized sample preparation process preserves your proteins
Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
Faster run times—get separation of your proteins in as little as 35 minutes
Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer (NP0007) and NuPAGE Sample Reducing Agent (NP0004). Use NuPAGE Antioxidant (NP0005) in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer (NP0002) to better resolve small proteins or NuPAGE MOPS SDS Running Buffer (NP000102) to resolve medium- to large-size proteins.

NuPAGE Bis-Tris Midi gels can be used with the XCell SureLock Midi Cell gel apparatuses (WR0100) or conveniently with the Bio-Rad Criterion Cell using our adapters (WA0999).

For transfer of proteins to a membrane, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device (IB21001). Alternatively, traditional wet transfer can be performed using the Bio-Rad Criterion Cell using NuPAGE Transfer buffer (NP00061).

For Research Use Only. Not for use in diagnostic procedures.

Figures