Please note that at certain times of the year Bis-Tris protein gel boxes may arrive inside insulated coolers containing ambient temperature gel packs.

Invitrogen™

NuPAGE™ 10% Bis-Tris Midi Protein Gels, 26-well, w/adapters

Catalog number: WG1203A

Related applications:

Protein Electrophoresis & Western Blotting

Description

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity. These precast gels are ideal for applications where protein integrity is crucial. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important. Also use NuPAGE gels to provide optimal results during day-to-day use.

Learn more about our NuPAGE Bis-Tris gels >

View migration charts ›

Features of NuPAGE Bis-Tris gels:
• Better protein integrity—optimized sample preparation process preserves your proteins
• Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
• Faster run times—get separation of your proteins in as little as 35 minutes
• Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer to better resolve small proteins or NuPAGE MOPS SDS Running Buffer to resolve medium- to large-size proteins.

NuPAGE Bis-Tris Midi gels can be used with the XCell SureLock Midi Cell gel apparatuses or conveniently with the Bio-Rad Criterion Cell using our adapters.

For transfer of proteins to a membrane, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device. Alternatively, traditional wet transfer can be performed using the Bio-Rad Criterion Cell using NuPAGE Transfer buffer.

Related links
Overview of 1D Protein Electrophoresis

For Research Use Only. Not for use in diagnostic procedures.

Figures

Bio-Rad Criterion™ XT 4-12% Bis-Tris Gel w/MOPS.

NuPAGE® Novex® 4-12% Bis-Tris Midi Gel w/MOPS.

Specifications

Adapters:	Includes Adapters
For Use With (Equipment):	Bio-Rad Criterion™ (with adapters),XCell4 SureLock™ Midi
Gel Percentage:	10 %
Gel Size:	Midi (8 cm x 13 cm)
Gel Type:	Bis-Tris Gel
Mode of Separation:	Molecular Weight
Pipette Compatibility:	Standard multi-channel pipettor
Product Line:	Novex™,NuPAGE®
Product Size:	10 gels (1 box)
Quantity:	10 gels
Recommended Applications:	Denaturing
Sample Loading Volume:	up to 15 µL
Separation Range:	15 to 260 kDa (MOPS buffer),3.5 to 160 kDa (MES buffer)
Shipping Condition:	Room Temperature
System:	NuPAGE® Novex®
Thickness:	1.0 mm
Well Format:	1D Well
Wells:	26 wells