NuPAGE™ Bis-Tris Midi Protein Gels, 4 to 12%, 1.0 mm
Please note that at certain times of the year Bis-Tris protein gel boxes may arrive inside insulated coolers containing ambient temperature gel packs.
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NuPAGE™ Bis-Tris Midi Protein Gels, 4 to 12%, 1.0 mm
Invitrogen™

NuPAGE™ Bis-Tris Midi Protein Gels, 4 to 12%, 1.0 mm

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity.
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Catalog NumberWells
WG1401BOX12 + 2-well
WG1401A12 + 2-well, w/adapters
WG1402BOX20-well
WG1402A20-well, w/adapters
WG1403BOX26-well
WG1403A26-well, w/adapters
Catalog number WG1401BOX
Price (USD)/ 10 gels (1 box)
203.65
Online exclusive
216.00 
Save 12.35 (6%)
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Add to cart
Wells:
12 + 2-well
Price (USD)/ 10 gels (1 box)
203.65
Online exclusive
216.00 
Save 12.35 (6%)
Add to cart
NuPAGE™ Bis-Tris Midi Protein Gels, 4 to 12%, 1.0 mm
Catalog numberWG1401BOX
Price (USD)/ 10 gels (1 box)
203.65
Online exclusive
216.00 
Save 12.35 (6%)
-
Add to cart
Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity. These precast gels are ideal for applications where protein integrity is crucial. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important.

Features of NuPAGE Bis-Tris gels:
Better protein integrity—optimized sample preparation process preserves your proteins
Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
Faster run times—get separation of your proteins in as little as 35 minutes
Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer (NP0007) and NuPAGE Sample Reducing Agent (NP0004). Use NuPAGE Antioxidant (NP0005) in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer (NP0002) to better resolve small proteins or NuPAGE MOPS SDS Running Buffer (NP000102) to resolve medium- to large-size proteins.

NuPAGE Bis-Tris Midi gels can be used with the XCell SureLock Midi Cell gel apparatuses (WR0100) or conveniently with the Bio-Rad Criterion Cell using our adapters (WA0999).

For transfer of proteins to a membrane, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device (IB21001). Alternatively, traditional wet transfer can be performed using the Bio-Rad Criterion Cell using NuPAGE Transfer buffer (NP00061).

For Research Use Only. Not for use in diagnostic procedures.
Specifications
AdaptersNo Adapters
For Use With (Equipment)XCell4 SureLock Midi-Cell, SureLock Tandem Midi Gel Tank
Gel Percentage4 to 12%
Gel SizeMidi
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 45 μL + 15 μL
Separation Range3.5 to 260 kDa
Separation TypeDenaturing
Shelf Life12 Months
Wells12 + 2-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)13 cm
Unit Size10 gels (1 box)