NuPAGE™ 3-8% Tris-Acetate Midi Protein Gels, 26-well, w/adapters

Catalog number:  WG1603A

Invitrogen™  Related applications: Protein Gel Electrophoresis

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Description

Invitrogen NuPAGE 3–8% Tris-Acetate Midi-Gels provide excellent separation of large molecular weight proteins. NuPAGE Tris-Acetate Midi Gels are high performance polyacrylamide gels that simulate the denaturing or the native conditions of the traditional laemmli system. Its unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of proteins compared to traditional Tris-glycine SDS-PAGE gels. Adapters are included with these gels that allow the gels to be run on a Bio-Rad Criterion™ Cell.

Features of the NuPAGE Tris-Acetate gels:
• Separate a wide range of molecular weight proteins
• Superior protein band resolution and stability
• Preserve protein sample integrity using optimized sample preparation processes

Learn more about all of our NuPAGE Tris-Acetate gels >

NuPAGE Tris-acetate gels are made with high-purity, strictly quality-controlled reagents. They contain tris base, acetic acid, acrylamide, bis-acrylamide, TEMED, APS, and highly purified water.

Run your proteins in native or denatured form
NuPAGE 3–8% Tris-Acetate Midi Gels gels do not contain SDS and so can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using NuPAGE LDS Sample Buffer and NuPAGE Tris-Acetate SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer. The gels can be run using our XCell4 SureLock Midi-Cell or conveniently with the Bio-Rad Criterion Cell using our adapters.

For transfer of proteins to a membrane, we recommend using the NuPAGE Transfer Buffer. Rapid semi-dry transfer can be done using the Pierce Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device. Alternatively, traditional wet transfer can be performed using the Bio-Rad Criterion Cell.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Adapters: Includes Adapters
For Use With (Equipment): Bio-Rad Criterion™ (with adapters), XCell4 SureLock™ Midi
Gel Percentage: 3-8 %
Gel Size: Midi (8 cm x 13 cm)
Gel Type: Tris-Acetate Gel
Mode of Separation: Molecular Weight
Product Line: Novex™, NuPAGE®
Product Size: 10 gels (1 box)
Quantity: 10 gels
Sample Loading Volume: up to 15 µL
Separation Range: 40 to 500 kDa (TA buffer)
System: NuPAGE® Novex®
Thickness: 1.0 mm
Well Format: 1D Well
Wells: 26 wells
Shipping Condition: Wet Ice

Contents & storage

One box contains 10 gels. Store in refrigerator (2–8°C). Do not freeze. Shelf life is 6 months.

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