NuPAGE™ Novex™ 3-8% Tris-Acetate Midi Protein Gels, 26-well, w/adapters

Catalog number: WG1603A

Invitrogen™  Related applications: Protein Gel Electrophoresis

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Description

NuPAGE® Novex 3-8% Tris-Acetate Midi-Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than the Tris-Glycine gel system.

NuPAGE® Novex Tris-Acetate Midi-Gels (8 x 13 cm) can run up to 26 samples. The NuPAGE Midi-Gels run in the XCell4 Surelock™ Midi-Cell and, with a Midi Gel Adapter, can run in a Bio-Rad Criterion Cell.

Formulation: NuPAGE® Novex Tris-Acetate gels are made with high-purity, strictly quality-controlled reagents: Tris base, acetic acid, acrylamide, bis-acrylamide, TEMED, APS, and highly purified water. They do not contain SDS.

Recommended Buffers: Run NuPAGE® Novex Tris-Acetate gels with Novex® Tris-Acetate SDS Running Buffer. To ensure good sample reduction and band resolution, use NuPAGE® Sample Preparation Reagents with these gels. The use of NuPAGE® Transfer Buffer provides optimal conditions for transfer of proteins to nitrocellulose, PVDF, or nylon membranes for subsequent analysis. For native running conditions, the Tris-Glycine Native Running and Sample Buffers should be used with NuPAGE® Tris-Acetate gels.

12+2 wells
Maximum sample volume/well 45 µl sample + 15 µl standards
Maximum protein load Coomassie® Stain 0.7 µg/band
Maximum protein load Silver Stain 1.4 ng/band

20 wells
Maximum sample volume/well 25 µl
Maximum protein load Coomassie® Stain 0.5 µg/band
Maximum protein load Silver Stain 1 ng/band

26 wells
Maximum sample volume/well 15 µl
Maximum protein load Coomassie® Stain 0.35 µg/band
Maximum protein load Silver Stain 0.7 ng/band


Currently using: Recommended NuPAGE® Gel:
4% Tris-Glycine NuPAGE® 3-8% Tris-Acetate (+ TA Buffer)
6% Tris-Glycine NuPAGE® 3-8% Tris-Acetate (+ TA Buffer)
10% Tris-Glycine NuPAGE® 8% Bis-Tris (+ MOPS Buffer)
or NuPAGE® 10% Bis-Tris (+ MOPS Buffer)
12% Tris-Glycine NuPAGE® 10% Bis-Tris (+ MOPS Buffer)
4-12% Tris-Glycine NuPAGE® 3-8% Tris-Acetate (+ TA Buffer)
or NuPAGE® 4-12% Bis-Tris (+ MOPS Buffer)
4-20% Tris-Glycine NuPAGE® 4-12% Bis-Tris (+ MES Buffer)
8-16% Tris-Glycine NuPAGE® 4-12% Bis-Tris (+ MOPS Buffer)
10% Tricine NuPAGE® 8% Bis-Tris (+ MES Buffer)
or NuPAGE® 10% Bis-Tris (+ MES Buffer)
16% Tricine NuPAGE® 4-12% Bis-Tris (+ MES Buffer)
10-20% Tricine NuPAGE® 4-12% Bis-Tris (+ MES Buffer)

For more information on the benefits of NuPAGE neutral pH technology go to www.invitrogen.com/nupage
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Adapters: Includes Adapters
For Use With (Equipment): Bio-Rad Criterion™ (with adapters only), XCell4 SureLock™ Midi
Gel Percentage: 3-8 %
Gel Size: Midi (8 cm x 13 cm)
Gel Type: Tris-Acetate Gel
Mode of Separation: Molecular Weight
Product Size: 10 gels (1 box)
Quantity: 10 gels
Sample Loading Volume: 15 µL
Separation Range: 40 kDa to 500 kDa (TA Buffer)
System: NuPAGE® Novex®
Thickness: 1.0 mm
Well Format: 1D Well
Wells: 26 wells

Contents & storage

One box contains 10 gels. Store in refrigerator (2–8°C). Do not freeze. Shelf life is 6 months.

Documents

Manuals & protocols

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