The BenchPro™ 4100 Western Card is a disposable, single-use card that is inserted into the slot on top of the BenchPro™ 4100 Card Processing Station. Developed for immunodetection, the card holds the membrane to be processed in a Blot Holder. During operation, reagents are pumped from reagent bottles and vials located in the reagent tray, into the card and around the membrane. The order of reagents used and timing of protocol steps are based on the protocol selected.