Authorsde Anda FC, Pollarolo G, Da Silva JS, Camoletto PG, Feiguin F, Dotti CG
JournalNature
PubMed ID16079847
'Neuronal polarization occurs shortly after mitosis. In neurons differentiating in vitro, axon formation follows the segregation of growth-promoting activities to only one of the multiple neurites that form after mitosis. It is unresolved whether such spatial restriction makes use of an intrinsic program, like during C. elegans embryo polarization, or ... More
Intracellular localization of type III-delivered Pseudomonas ExoS with endosome vesicles.
AuthorsZhang Y, Deng Q, Barbieri JT
JournalJ Biol Chem
PubMed ID17311921
'ExoS (453 amino acids) is a bi-functional type III cytotoxin produced by Pseudomonas aeruginosa. Residues 96-219 include the Rho GTPase-activating protein (RhoGAP) domain, and residues 234-453 include the 14-3-3-dependent ADP-ribosyltransferase domain. Earlier studies also identified an N-terminal domain (termed the membrane localization domain) that comprises residues 51-77 and includes a ... More
Mixed gastric- and intestinal-type metaplasia is formed by cells with dual intestinal and gastric differentiation.
AuthorsNiwa T, Ikehara Y, Nakanishi H, Tanaka H, Inada K, Tsukamoto T, Ichinose M, Tatematsu M
JournalJ Histochem Cytochem
PubMed ID15637340
'We have proposed to divide intestinal metaplasia (IM) into two categories, i.e., a mixed gastric and intestinal (GI) type, and a solely intestinal (I) type, based on the residual gastric phenotype cells. The GI-mixed-type IM can be identified by the presence of both cells with either gastric or intestinal phenotypes ... More
Interaction codes within the family of mammalian Phox and Bem1p domain-containing proteins.
AuthorsLamark T, Perander M, Outzen H, Kristiansen K, Øvervatn A, Michaelsen E, Bjørkøy G, Johansen T,
JournalJ Biol Chem
PubMed ID12813044
'The Phox and Bem1p (PB1) domain constitutes a recently recognized protein-protein interaction domain found in the atypical protein kinase C (aPKC) isoenzymes, lambda/iota- and zeta PKC; members of mitogen-activated protein kinase (MAPK) modules like MEK5, MEKK2, and MEKK3; and in several scaffold proteins involved in cellular signaling. Among the last ... More
Quantitation of microparticles released from coated-platelets.
AuthorsDale GL, Remenyi G, Friese P,
JournalJ Thromb Haemost
PubMed ID16102115
'Dual agonist stimulation of platelets with thrombin and convulxin results in generation of coated-platelets, a sub-population of cells known formerly as COAT-platelets (collagen and thrombin). Coated-platelets retain several procoagulant proteins on their surface and express phosphatidylserine (PS). In this report, we utilize a new methodology to demonstrate that coated-platelets also ... More
Expression of the integrin subunit alpha8 in murine lung development.
AuthorsWagner TE, Frevert CW, Herzog EL, Schnapp LM
JournalJ Histochem Cytochem
PubMed ID14500699
'The complex interplay between cells and extracellular matrix (ECM) proteins is critical for lung development. Integrins are key modulators of this interaction. The integrin subunit alpha 8 associates with the beta(1)-subunit to form an RGD-binding integrin. We previously showed that, in adult lung, alpha 8 is expressed in contractile interstitial ... More
Vectorial insertion of apical and basolateral membrane proteins in polarized epithelial cells revealed by quantitative 3D live cell imaging.
AuthorsHua W, Sheff D, Toomre D, Mellman I
JournalJ Cell Biol
PubMed ID16567501
Although epithelial cells are known to exhibit a polarized distribution of membrane components, the pathways responsible for delivering membrane proteins to their appropriate domains remain unclear. Using an optimized approach to three-dimensional live cell imaging, we have visualized the transport of newly synthesized apical and basolateral membrane proteins in fully ... More
Zinc finger protein Wiz links G9a/GLP histone methyltransferases to the co-repressor molecule CtBP.
AuthorsUeda J, Tachibana M, Ikura T, Shinkai Y
JournalJ Biol Chem
PubMed ID16702210
G9a is a SET-domain mammalian histone methyltransferase responsible for mono- and dimethylation of lysine 9 in histone H3 (H3K9) at euchromatic regions. Recently we reported that G9a forms a stoichiometric heteromeric complex with another SET-domain-containing molecule, GLP/Eu-HMTase1. Although G9a and GLP can independently methylate H3K9 in vitro, G9a/GLP heteromeric formation ... More
Distinct endosomal compartments in early trafficking of low density lipoprotein-derived cholesterol.
AuthorsSugii S, Reid PC, Ohgami N, Du H, Chang TY
JournalJ Biol Chem
PubMed ID12721287
We previously studied the early trafficking of low density lipoprotein (LDL)-derived cholesterol in mutant Chinese hamster ovary cells defective in Niemann-Pick type C1 (NPC1) using cyclodextrin (CD) to monitor the arrival of cholesterol from the cell interior to the plasma membrane (PM) (Cruz, J. C., Sugii, S., Yu, C., and ... More
Plasma membrane topology of syncytial domains of herpes simplex virus type 1 glycoprotein K (gK): the UL20 protein enables cell surface localization of gK but not gK-mediated cell-to-cell fusion.
AuthorsFoster TP, Alvarez X, Kousoulas KG
JournalJ Virol
PubMed ID12477855
Most spontaneously occurring mutations that cause extensive herpes simplex virus type 1 (HSV-1)-induced cell fusion are single amino acid changes within glycoprotein K (gK). Despite the strong genetic association of gK with virus-induced cell fusion, its direct involvement in cellular membrane fusion has been controversial, largely due to previously unsuccessful ... More