The ZOOM™ IPGRunner™ System is the first system to offer oil-free, trouble-free 2D electrophoresis in a mini-gel format. First dimension separation isoelectric focusing (IEF) is complete in less than three hours. The mini-gel design of the system is easy to handle, eliminates mineral oil overlays, and allows processing of up to 12 samples at once for high-throughput usage.
The ZOOM™ IPGRunner™ System consists of three main components:
• The ZOOM™ IPGRunner™ Mini-Cell. (Figure 1) includes a high-voltage electrophoresis core and lid assembly that fits a mini-cell chamber identical to the XCell SureLock™ mini-cell. The mini-cell holds two ZOOM™ IPGRunner™ Cassettes.
• The ZOOM™ IPGRunner™ Cassettes (Figure 2) are provided ready to use and self-contained, eliminating oil overlays for convenient sample rehydration and IEF. Each cassette holds up to six ZOOM™ Strips.
• The ZOOM™ Strips are 7 cm long and contain a thin layer of polyacrylamide gel that includes a fixed pH gradient. Each ZOOM™ Strip is clearly labeled with a unique identifying number, pH range, and orientation marks (Figure 3). ZOOM™ Strips are supplied attached to a tri-fold card (Figure 4) for easy access and removal.
Quick and Simple Set-up Procedure
Figure 5 demonstrates how easy and fast it is to load samples and set up the ZOOM™ IPGRunner™ System for IEF. Once IEF is complete, the ZOOM™ IPGRunner™ Mini-Cell can be used with NuPAGE™ and Novex™ ZOOM™ gels to complete SDS-PAGE analysis in 40 minutes.
For Research Use Only. Not for use in diagnostic procedures.